TABLE 1.

Strains and plasmids used in this study

Strain or plasmidDescriptionReference or source
S. oneidensis strains
    MR-1Lake Oneida isolate23
    SR660MR-1 ΔcyaAThis study
    SR945MR-1 ΔcyaBThis study
    SR672MR-1 ΔcyaCThis study
    SR665MR-1 ΔcyaA ΔcyaCThis study
    SR946MR-1 ΔcyaA ΔcyaB ΔcyaCThis study
    SR694MR-1 ΔcrpThis study
    SR956SR946 with cyaA in pJB3Cm6, CmrThis study
    SR957SR946 with cyaB in pJB3Cm6, CmrThis study
    SR958SR946 with cyaC in pJB3Cm6, CmrThis study
E. coli strains
    EC100mcrA Δ(mrr-hsdRMS-mcrBC) recA1Epicenter Technologies
    EC100D+E. coli EC100 derivative, pir+Epicenter Technologies
    β2155pir::RP4, Kmr5
    SP850ΔcyaA1400 (::kan)31
    E299E. coli SP850 with pJB3Cm6, Kmr CmrThis study
    E300E. coli SP850 with cyaA in pJB3Cm6, Kmr CmrThis study
    E301E. coli SP850 with cyaC in pJB3Cm6, Kmr CmrThis study
    E347E. coli SP850 with Po-cyaB in pJB3Cm6, Kmr CmrThis study
    E456E. coli SP850 with cyaB in pJB3Cm6, Kmr CmrThis study
Plasmids
    pJB3Cm6Cloning and sequencing vector, Cmr2
    pDS3.1R6K ori Gmr sacB38
    pPR9TTPromoter probe vector, lacZ Cmr Apr30
    pKB1cyaC in pJB3Cm6, CmrThis study
    pMC2cyaA in pJB3Cm6, CmrThis study
    pMC3cyaB fused to operon promoter (Po) in pJB3Cm6This study
    pMC4cyaB and 300 bp of upstream DNA in pJB3Cm6This study
    pDS10dms promoter fused to lacZ in pPR9TTThis study
    pDS11fccA promoter fused to lacZ in pPR9TTThis study
    pDS12cyaC promoter fused to lacZ in pPR9TTThis study
    pDS13cyaA promoter fused to lacZ in pPR9TTThis study