TABLE 3.

Genes with altered expression profiles in the Δcrp mutant compared to the wild type for cells grown under O2-limited conditions

LocusGeneFunction or descriptionΔcrp mutant/wild type ratioa
Upregulated genes
    Heme biosynthesis
        SO_0027hemG-2Protoporphyrinogen oxidase0.05
        SO_0435hemEUroporphyrinogen decarboxylase0.22
        SO_2109hemH-1Ferrochelatase0.34
        SO_2587hemB-1Delta-aminolevulinic acid dehydratase0.20
        SO_3720hemG-3Protoporphyrinogen oxidase0.30
        SO_4208hemB-2Delta-aminolevulinic acid dehydratase0.25
    Respiratory functions
        SO_0396frdCQuinol:fumarate reductase0.13
        SO_0848napAPeriplasmic nitrate reductase0.30
        SO_0902nqrANa-translocating NADH-quinone reductase subunit A0.21
        SO_0903nqrBNa-translocating NADH-quinone reductase subunit B0.14
        SO_0904nqrCNa-translocating NADH-quinone reductase subunit C0.11
        SO_0970fccAPeriplasmic fumarate reductase0.14
        SO_2912pflBPyruvate formate lyase0.17
        SO_2913pflAPyruvate formate lyase 1 activating subunit0.20
        SO_3922fdhFormate dehydrogenase, cytochrome b0.32
        SO_4591cymAMembrane-anchored tetraheme c cytochrome0.22
    Regulatory functions
        SO_0490Transmembrane transcriptional regulator0.19
        SO_0940Transmembrane transcriptional regulator0.07
        SO_1415Transcriptional regulator, TetR family0.12
        SO_3059Sigma 54 transcriptional regulator, Fis family0.28
        SO_3538hlyUTranscriptional regulator0.06
        SO_3627Transcriptional regulator, TetR family0.14
Downregulated genes
    SO_0852Type IV pilin, putative4.76
    SO_1329cyaCAdenylate cyclase2.08
    SO_4312cyaAAdenylate cyclase3.97
    SO_4609coxCaa3-type cytochrome c oxidase, subunit III2.68
  • a Ratio of the mean hybridization signal intensity for the crp mutant to the mean hybridization signal intensity for the wild type. Values greater than 1 indicate an increase in the mRNA level in the mutant; values less than 1 indicate a decrease.