TABLE 6.

β-Galactosidase activities for dms and fccA promoter-lacZ fusions under anaerobic conditions in wild-type and mutant strains of S. oneidensis MR-1

Strainβ-Galactosidase activity (Miller units)a
dms promoterbfccA promoterc
MR-1237.1 ± 23.9341 ± 6.2
ΔcyaC mutant61.9 ± 1.7242.8 ± 5.2
ΔcyaAC mutant19.9 ± 0.755.9 ± 1.4
Δcrp mutant25.0 ± 5.371.4 ± 1.8
  • a The β-galactosidase activity in MR-1 carrying pPR9TT without an insert was 10 Miller units. The data are means ± standard deviations of three independent experiments.

  • b Cultures were incubated anaerobically with DMSO for 2 h.

  • c Cultures were incubated anaerobically with fumarate for 2 h.