TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant propertiesSource or reference
Strains
    E. coli strains
        DH5αF′ endA1 hsdR17 supE44 thi-1 recA1 gyrA96 relA1 Δ(argF-lacZYA)U169 (φ80dlacΔM15)Promega
        CC118 (λ pir)Δ(ara-leu) araD ΔlacX74 galE galK phoA20 thi-1 rpsE rpoB argE(Am) recA1 λ pir21
        S17-1 (λ pir)Tpr Smr recA thi pro (rK mK+) RP4:2-Tc:MuKm Tn7 λ pir10
    V. cholerae strains
        FY_Vc_1Vibrio cholerae O1 El Tor A1552, smooth variant, Rifr64
        FY_Vc_22ΔlacZ Rifr9
        FY_Vc_2497ΔcpxA RifrThis study
        FY_Vc_2417ΔenvZ RifrThis study
        FY_Vc_3349ΔoscR RifrThis study
        FY_Vc_2578ΔrpoS ΔlacZ RifrThis study
        FY_Vc_2919ΔhapR ΔlacZ Rifr13
        FY_Vc_2922ΔvpsT ΔlacZ Rifr13
        FY_Vc_2874ΔvpsR ΔlacZ Rifr13
        FY_Vc_2459Δcrp ΔlacZ Rifr13
        FY_Vc_616vpsLp-lacZ RifrThis study
        FY_Vc_3362vpsLp-lacZ ΔoscR RifrThis study
        FY_Vc_3771vpsLp-lacZ ΔVC1353 RifrThis study
        FY_Vc_3773vpsLp-lacZ ΔoscR ΔVC1353 RifrThis study
        FY_Vc_3792vpsLp-lacZ VCA0030::pGP704 Rifr AprThis study
        FY_Vc_3796vpsLp-lacZ ΔoscR VCA0030::pGP704 Rifr AprThis study
        FY_Vc_4140ectAp-lacZ RifrThis study
        FY_Vc_4143VC2037p-lacZ RifrThis study
        FY_Vc_237FY_Vc_1 mTn7-gfp Gmr4
        FY_Vc_3065ΔcpxA mTn7-gfp Rifr GmrThis study
        FY_Vc_3068ΔenvZ mTn7-gfp Rifr GmrThis study
        FY_Vc_1739ΔvpsT mTn7-gfp Rifr GmrThis study
        FY_Vc_2407ΔvpsR mTn7-gfp Rifr GmrThis study
        FY_Vc_3359ΔoscR mTn7-gfp Rifr GmrThis study
Plasmids
    pBAD102/D/lacZControl vector containing lacZ; Apr KmrInvitrogen
    pGP704mob/oriT Apr40
    pFY-656pGP704::VCA0030 AprThis study
    pGP704-sacB28pGP704 derivative; mob/oriT sacB Apr9
    pFY-591pGP704-sacB28::ΔcpxA AprThis study
    pFY-315pGP704-sacB28::ΔenvZ AprThis study
    pFY-624pGP704-sacB28::ΔoscR AprThis study
    pFY-393pGP704-sacB28::ΔVC1353 AprThis study
    pFY-217pGP704-sacB28::vpsLp-lacZ transcriptional fusion; AprThis study
    pFY-714pGP704-sacB28::ectAp-lacZ transcriptional fusion; AprThis study
    pFY-707pGP704-sacB28::VC2037p-lacZ transcriptional fusion; AprThis study
    pRS415Promoterless lacZ cloning vector for transcriptional fusion studies; Apr55
    pFY-49pRS415 vpsL promoter; Apr9
    pACYC177Low-copy-number cloning vector; AprNew England Biolabs
    pFY-622pACYC177::oscRThis study
    pUX-BF13oriR6K helper plasmid; provides the Tn7 transposition function in trans; mob/oriT Apr1
    pMCM11pGP704::mTn7-gfp Gmr AprG. Schoolnik