TABLE 4.

COMSTAT analysis of biofilm structures formed by V. cholerae wild-type, ΔvpsR, and ΔvpsT strains in 0.2 M and 0.5 M NaCl-containing 2% LBa

Time (h)StrainNaCl concn (M)Total biomass (μm3/μm2)Thickness (μm)Roughness coefficient
AvgMaximum
24Wild type0.28.9 (1.0)9.9 (0.7)15.9 (1.0)0.11 (0.02)
0.59.0 (1.2)10.0 (1.5)15.4 (1.2)0.10 (0.02)
ΔvpsR0.22.8 (1.5)4.2 (2.3)7.5 (0.5)0.13 (0.07)
0.52.1 (1.5)2.8 (1.7)5.9 (2.5)0.74 (0.74)
ΔvpsT0.20.9 (1.6)1.5 (2.6)4.9 (5.0)0.63 (0.94)
0.52.2 (1.7)3.5 (2.8)11.6 (1.3)0.13 (0.10)
48Wild type0.211.9 (1.6)16.3 (1.0)28.7 (2.8)0.14 (0.04)
0.517.3 (2.1)18.7 (1.2)28.9 (1.7)0.10 (0.02)
ΔvpsR0.22.9 (2.3)4.5 (3.6)9.6 (0.7)0.08 (0.06)
0.53.0 (2.9)3.9 (3.4)8.3 (0.9)0.88 (0.85)
ΔvpsT0.21.4 (2.2)2.3 (3.6)7.8 (5.1)0.73 (0.98)
0.52.6 (2.0)4.3 (3.4)9.8 (4.7)0.45 (0.76)
72Wild type0.215.4 (6.4)21.7 (8.2)39.5 (12.9)0.37 (0.53)
0.528.4 (1.6)25.3 (7.8)44.9 (2.2)0.11 (0.02)
ΔvpsR0.24.4 (0.8)6.1 (1.3)11.0 (1.3)0.16 (0.02)
0.52.5 (1.4)4.9 (2.1)9.6 (4.8)0.61 (0.68)
ΔvpsT0.20.4 (1.1)0.8 (2.3)6.0 (9.7)1.31 (0.96)
0.52.4 (2.4)2.4 (3.5)18.3 (3.1)0.13 (0.13)
  • a Values presented are means of data from at least five z-series image stacks. The numbers in parentheses indicate standard deviations.