TABLE 1.

Resistance of E. coli cells harboring a plasmid carrying evgA, yhiUV, or emrKY

CompoundMIC (μg/ml)a for strain:
KAM3KAM3/ pUCAKAM3ΔyhiUVKAM3ΔyhiUV/ pUCAKAM3/ pUCyhiUVKAM3/ pQE30emrKYbKAM3/ pUCA-D52AKAM3/ pUCA-D54A
Doxorubicin3.132003.133.13253.1325>200
Erythromycin3.13253.133.13253.133.1350
Crystal violet1.5612.51.561.563.131.563.1312.5
Rhodamine 6G6.251006.256.251006.256.25100
Benzalkonium3.13253.133.136.253.133.1325
SDS100400100100200100100>400
Deoxycholate1,250>40,0001,2505,0005,00010,0001,250>40,000
  • a Boldface indicates values significantly different from control values. Drug resistance of cells was determined by a sequential twofold dilution method on YT (24) agar plates as previously described (15).

  • b IPTG (1 mM) was added to agar plates.