TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant genotype or phenotypeSource or reference
E. coli strains
    MG1655 DH5αWild type, donor strain F φ80d lacZΔM15 Δ(lacZYA-argF)U169 deoR recA1 endA1 hsdR17(rK mK) gal-phoA supE44 λ thi-1 gyrA96 relA1
    ABLE Clac(lacZω) [Kanr McrA McrCB, McrF Mrr hsdR(rK mK)][F′ proAB lacIqZΔM15 Tn10(Tetr)]Stratagene, Inc.
    TOP10F′F′[lacIq Tn10(Tetr)] mcrA Δ(mrr-hsdRMS-mcrBC) φ80 lacZΔM15ΔlacX74 deoR recA1 araD139 Δ(ara-leu)7697 galU galK rpsL(Strr) endA1 nupGInvitrogen, Inc.
    CG3014W 3110 galE, resistant to phages T1, T5, carries Tn10(Tetr), wild type4
    CG3010As above, but groEL44, Tetr4
    CG3013As above, but groES619, Tetr4
Plasmids
    pBAD24Ampr, l-arabinose-inducible expression vector6
    pBAD24-sugEAmpr, pBAD24 carrying sugEThis study
    pCR2.1 (pCR2.1 TOPO)Ampr, Kanr, PCR cloning vectorInvitrogen, Inc.
    pCR2.1-sugEAmpr, Kanr, pCR 2.1 carrying the sugE gene plus 134-bp upstream promoter region of sugEThis study