TABLE 2.

Primers used in this study

NameSequencea
PilSk/orevGCATGGATCCGTCGTTGGCTTGGCGCATGGC
PilSk/ofwdGCATGGATCCGCAGCCTGCAACATTTGTTAGCCG
pilRRevACGTGTCGACCATATTGATTATTCAATATTCAAGCGTTCC
pilRFwdACGTGAATTCATGACCAAACACAGTTTGAATCGCC
pilRk/oFwdACGTACGCGTGCGAAAGCGGTTCGGGTAAAGAG
pilRk/oRevACGTACGCGTCCGAAATATAGACAGGCACAACGGC
ΔpilA-12fwdCAAATGGCATGCACTCTAATACCAAGTAAGGCAAGTAACACAGC
ΔpilA-12revGCTGTGTTACTTGCCTTACTTGGTATTAGAGTGCATGCCATTTG
ΔACA#1FwdATCGTGAGATCTGTGACTGAGGTCTTGGCATGCACTCTGCTACCAAGTAAGGCAAGTAAC
ΔACA#1RevATCGTGAGATCTGTGGATCGGCTAGAATGGTATTTTATTAAACTGCCTTTTTTTGTCAGTCGCAC
ΔACA#2FwdATCGTGACTAGTGCACTGCGTCGGCGGCACGTTGATAAGCGAATTGCACCCAAATTG
ΔACA#1RevATCGTGACTAGTGGTCTAGCGTCGAAAATAGCGCGCTGATTGAAGCCTGCAATCTG
SRLFwdATGCATGGTACCAGAAAGCGAATTGAACGCGTTGC
SRLRevATGCATGTCGACGCTTTCACGTCTTCGGGCAGC
pilRDSBFATCGGGATCCTTTTTGATTTGGGTATAAAAGGGTATGCCGTAGG
pilRDSBRATCGGGATCCTTTGGACAAAAGGGCAAAAAGCAGCC
PilinFΔSSTACGGGATCCAGATTACACCAAACGTTCACGCGTATCTGA
PilinRTACGGAATTCTTAGCCACCATTGGTAGCGGTAGTAGTGCC
pilA1pdr-6famFAM-CTCAGTCAAAGCACTTTTCACG
pilA1pdrCTCAGTCAAAGCACTTTTCACG
pilA1pdfGAGCAGGGAACAGATTGCAGG
Pilin Region FwdACGTGAATTCAAGCGCGTATGCCGTGCGAC
Pilin Region S3GCCGATTATGATGCCGATGGC
pilA1 RT-leftCAAACGTTCACGCGTATCTG
pilA1 RT-rightGGCCATGCATTGTTAGAAGC
ftsZ RT-leftCCAGAGCGAACCAAAGTCTC
ftsZ RT-rightAAGCTATACTCGCCCTGCTG
pillR RT-leftGTTGATGTCCGCATTGTGTC
pilR RT-rightGGCGATAGAACAGGTCTTGG
moxR RT-leftTTTATGTTGGCGGACGAAAT
moxR RT-rightCGTCCACCGATACTTGACCT
raiA-rightGCGGTTACCTTGTCGGTAGA
raiA-leftTCTTCCACTGCTTCAACGTG
  • a FAM, 6-carboxyfluorescein.