TABLE 1.

Strains used in this study

StrainDescriptionReference or source
269-492K. kingae isolate from St. Louis Children's Hospital19
KK01Nonspreading/noncorroding variant of strain 269-49219
KK03Spreading/corroding variant of strain 269-49219
269-492 pilFStrain 269-492 with an aphA3 insertion in pilF19
269-492 pilA1Strain 269-492 with an aphA3 insertion in pilA118
269-492 recJStrain 269-492 with an aphA3 insertion in recJ18
KK01 recJStrain KK01with an aphA3 insertion in recJThis work
KK03 recJStrain KK03 with an aphA3 insertion in recJThis work
269-492 rpoNStrain 269-492 with an aphA3 insertion in rpoNThis work
269-492 Δ−12Strain 269-492 with an aphA3 insertion in recJ and replacement of the σ54 −12 DNA GC binding region with AAThis work
269-492 pilSStrain 269-492 with an aphA3 insertion in pilSThis work
KK01 pilSStrain KK01 with an aphA3 insertion in pilSThis work
KK03 pilSStrain KK03 with an aphA3 insertion in pilSThis work
269-492 pilRStrain 269-492 with an aphA3 insertion in pilRThis work
KK01 pilRStrain KK01 with an aphA3 insertion in pilRThis work
KK03 pilRStrain KK03 with an aphA3 insertion in pilRThis work
269-492/ACA#1Strain 269-492 with an aphA3 insertion in recJ and replacement of ACA#1 repetitive element with random DNAThis work
KK01 ACA#1Strain KK01 with an aphA3 insertion in recJ and replacement of ACA#1 repetitive element with random DNAThis work
KK03 ACA#1Strain KK03 with an aphA3 insertion in recJ and replacement of ACA#1 repetitive element with random DNAThis work
269-492/ACA#2Strain 269-492 with an aphA3 insertion in recJ and replacement of ACA#2 repetitive element with random DNAThis work
KK01 ACA#2Strain KK01 with an aphA3 insertion in recJ and replacement of ACA#2 repetitive element with random DNAThis work
KK03 ACA#2Strain KK03 with an aphA3 insertion in recJ and replacement of ACA#2 repetitive element with random DNAThis work
269-492/ACA#1/ACA#2Strain 269-492 with an aphA3 insertion in recJ and replacement of ACA#1/ACA#2 repetitive elements with random DNAThis work
KK01 ACA#1/ACA#2Strain KK01 with an aphA3 insertion in recJ and replacement of ACA#1/ACA#2 repetitive elements with random DNAThis work
KK03 ACA#1/ACA#2Strain KK03 with an aphA3 insertion in recJ and replacement of ACA#1/ACA#2 repetitive elements with random DNAThis work
DH5αE. coli F φ80dlacZΔM15 Δ(lacZYA-argF)U169 deoR recA1 endA1 hsdR17(rK mK+) phoA supE44 thi-1 gyrA96 relA129
DH5α MalC2DH5α containing pMalC2, which expresses MBPThis work
DH5α MAL-PilRDH5α containing pMalC2:pilR, which expresses MBP-PilRThis work
DH5α pilA1:ΔSSDH5α containing pHAT10/pilA1:ΔSS, which expresses HAT-PilA1ΔSSThis work