TABLE 2.

Proteomic analysis of the effects of mutation of rpfF on Burkholderia cenocepacia J2315

J2315 annotationaPredicted functionbGeneAbundance in the mutant relative to that in the wild typecMOWSE scored
BCAL3270Putative DnaK chaperone protein; NCBI-GI 206561609dnaK102
BCAM2759Putative minor pilin and initiator; NCBI-GI 206564597cblD89
BCAM0242Hypothetical protein; NCBI-GI 206562112++81
BCAM0791Major facilitator superfamily protein; NCBI-GI 206562648mfs87
BCAM0421LysR family regulatory protein; NCBI-GI 20656228496
BCAM0114Hypothetical protein; NCBI-GI 206561984++101
BCAM1600Hypothetical protein; NCBI-GI 206563449++125
BCAL0055Putative heavy metal resistance membrane ATPase; NCBI-GI 20655845985
BCAL0521Flagellar FliJ protein; NCBI-GI 206558922fliJ126
  • a Obtained from http://genome.jgi-psf.org/finished_microbes/burca/burca.home.html.

  • b Functions and gene identification (NCBI-GI) number according to NCBI at http://www.ncbi.nlm.nih.gov/.

  • c Relative abundance levels in the rpfF mutant and the J2315 wild type are represented as follows: −, present in wild type but absent in mutant; ++, present in wild type but elevated in mutant. Changes were seen in each of three replicate experiments.

  • d The Mascot software (Matrix Science, London) was used to identify the proteins on the basis of peptide mass fingerprinting. The software was set to allow up to two missed cleavages and a mass deviation of 50 ppm. Only protein predictions with a MOWSE score higher than 80 were considered significant.