Summary of proteome analysis, Northern hybridization analysis, and effect of protease inhibitors on the two-dimensional pattern

Spot no.CPE no.Product (homologous protein) (reference)PatternaDensitybNortherncEffect of protease inhibitord
Wild typeMutantL, AB
P1CPE19512′,3′-cyclic nucleotide 2′-phosphodiesteraseW726474P (0.26)+/−+/−
P2CPE0163Perfringolysin OW22,167732P (0.22)+/−
P3CPE1529Hypothetical protein (ydaL gene product from Bacillus subtilis [29.3%]) (17)W2,0170P (0.83)+/−+/−
P4CPE0846Cysteine protease (alpha-clostripain from Clostridium histolyticum [58.3%]) (7)WN.D.N.D.P (0.32)+/−
P5CPE0173CollagenaseW5300P (0.30)+/−+/−
P6CPE1231Probable surface protein (Aas surface protein from Staphylococcus saprophyticus [25.4%]) (13)W11,2430I (1.09)
P7CPE0220Hypothetical protein (chitinase A from Clostridium paraputrificum [38%]) (24)W1,1580I (1.04)+/−+/−
N1CPE1428ClpB protein (endopeptidase Clp ATP-binding chain B1 from Synechocystis sp. [55.8%]) (15)M04,780I (1.00)++
N2CPE0202Probable cell wall-binding protein (cell wall-binding protein from Bacillus halodurans [32.4%]) (31)M03,839N (1.51)++
N3CPE1785Iron-sulfur cofactor synthesis protein NifSM3,01513,371I (1.05)++
N4CPE1632RibokinaseM05,352I (1.08)++
N5CPE2297β-Hydroxybutyryl-coenzyme A dehydrogenase, NAD dependentM06,403I (1.00)+/−+/−
N6CPE1350Fructose-bisphosphate aldolaseM010,109N (1.46)++
N7CPE2408Elongation factor GM1,7923,525I (0.95)+/−+/−
N8CPE0278Conserved hypothetical protein (p45 from Listeria monocytogenes [26%]) (26)M08,698I (0.98)+/−+/−
  • a W, exclusive to the wild type, M, exclusive to the virR mutant.

  • b Values represent the ratio of spot density level (in ppm) to the total of all valid spots using PDQuest software (Bio-Rad). N.D., not determined.

  • c P, positively regulated; I, independently regulated; N, negatively regulated. Values represent the ratio of specific mRNA level in the mutant to that in the wild type.

  • d +/−, not altered; +, induced; −, reduced. L, leupeptin; A, antipain; B, benzamidine.