TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant genotype or phenotypeaReference or source
Strains
    P. aeruginosa
        PAO1Wild typeHolloway collection
        PAO1 lecA::lux lecA::luxCDABE genomic reporter fusion in PAO1 62
        PAO-P4Tn5-B21 lasR mutant derived from PAO1 lecA::luxThis study
        PAO-P8Tn5-B21 mutant derived from PAO1 lecA::luxThis study
        PAO-P9Tn5-B21 rpoS mutant derived from PAO1 lecA::luxThis study
        PAO-P10Tn5-B21 mvaT mutant derived from PAO1 lecA::luxThis study
        PAO-P19Tn5-B21 mutant derived from PAO1 lecA::luxThis study
        PAO-P34Tn5-B21 rhlR mutant derived from PAO1 lecA::luxThis study
        PAO-P47 mvaT chromosomal deletion mutant derived from PAO1This study
        PAO-P52Tn5-B21 clpA mutant derived from PAO1 lecA::luxThis study
        PAO-P69Tn5-B21 mutant derived from PAO1 lecA::luxThis study
    E. coli
        JM109 recA1 supE44 endA1 hsdR17 gyrA96 relA1 thi Δ(lac-proAB) F′ [traD36 proAB+lacIqlacZΔM15] 63
        S17-1 λpir thi pro hsdR hsdM + recA RP4-2-Tc::Mu-Km::Tn7 λpir 46
Plasmids
    pUC18Multicopy cloning vector derived from pBR322 carrying polylinker inserted into lacZα (Ampr) 54
    pUCP18Same as pUC18 but containing an additional 1.8-kb stabilizing fragment for maintenance in Pseudomonas spp. (Ampr) 44
    pDM4Suicide vector carrying the sacBR genes for sucrose sensitivity (Cmr) 26
    pSP73Cloning vectorPromega Corp.: Madison, Wis.
    pSUP102 Tn5-B21Transposable promoter probe; B21 in pSUP102 (Cmr Tetr) 47
    pSB1075AHL reporter plasmid; P. aeruginosa lasRI and luxCDABE from Photorhabdus luminescens (Ampr) 61
    pSB536AHL biosensor; ahyR"::luxCDABE in pAHP13 (Ampr) 51
    pUCmvaT1.6-kb BamHI-PstI PAOI chromosomal DNA fragment containing mvaT in pUC18This study
    pUCΔmvaTSame as pUC::mvaT except contains a 327-bp mvaT deletion in pUC18This study
    pDM4ΔmvaTpDM4 containing mvaT flanking regions and deletion-containing mvaT geneThis study
    pUCPmvaT1.6-kb PAO1 BamHI-PstI DNA insert containing mvaT gene in pUCP18This study
    pMW47.12-kb PstI PAO1 DNA insert (rhlRl) in pUCP18 23
  • a Ampr, Cmr, and Tetr stand for resistance to ampicillin, chloramphenicol, and tetracycline, respectively.