TABLE 2.

Quantitation of cell length and FtsZ rings after MinC/DicB-mediated filamentationa

PlasmidTime (min)R cellsR+ cellsR + R+ cells
% (n)Avg length (range) (μm)% (n)Avg length (range) (μm)L/R (μm)Avg length (range) (μm)L/R (μm)
pJE44 [PBAD::dicB]1020 (9)6.7 (3.2-20.0)80 (36)13.4 (4.4-33.5)5.512.0 (3.2-33.5)6.1
607 (2)21.9 (8.0-35.8)93 (25)15.1 (4.2-49.8)4.915.6 (4.2-49.8)5.4
12035 (9)39.0 (4.2-66.3)65 (17)30.3 (2.6-70.8)16.133.3 (2.6-70.8)27.1
18030 (17)51.4 (3.5-98.4)70 (39)11.0 (2.5-97.6)8.623.3 (2.5-98.4)26.0
pBAD33 (vector)016 (14)2.5 (1.9-3.5)84 (72)3.5 (1.9-15.9)2.73.4 (1.9-15.9)3.1
18022 (13)2.5 (1.8-3.8)78 (47)2.8 (1.9-4.5)2.72.7 (1.8-4.5)3.4
  • a Cells of strain PB147(λDR120) [ΔminDE(Plac::gfp-ftsZ)] harboring the indicated plasmid or vector were treated and analyzed as described in the text. Parameters were calculated separately for cells without fluorescent ring structures (R cells), cells with one or more rings (R+ cells), and all cells combined (R + R+ cells). n, number of cells examined. Any visible accumulation of fluorescence resembling a ring was counted as one.