Functionality and localization of GFP-MinC fusionsa

Protein (plasmid)LL1 (λDB182) [ΔminCDE(Plac::dicB)]LL1(λDR155) [ΔminCDE(Plac::minD)]LL1 (λDB175) [ΔminCDE(Plac::minDE)]
GFP-T-MinC(5-231) (pLL18)SepCbSepMbWTO
GFP-T-MinC(14-231) (pLL13)MinRMinRMinO/R
GFP-T-MinC(108-231) (pPC105)MinRMinRMinO/R
GFP-T-MinC(141-231) (pLL14)MinCMinCMinC
GFP-T-MinC(108-231)-H (pJE78)MinRMinRMinO/R
GFP-T-MinC(108-208)-H (pJE79)MinCMinCMinC
  • a Lysogens containing the indicated plasmids were grown for ≈5 h at 37°C in the presence of 100 μM IPTG to an OD600 of 0.1. Live cells were observed through fluorescence optics to determine the localization pattern of the GFP-MinC fusion (Loc.) and through phase-contrast optics to determine the division phenotype (Phen.). C, cytoplasmic; M, along the periphery of the cell; O, oscillating; R, stably associated with rings; O/R, transiently associated with rings during oscillation cycle. When grown in the absence of IPTG, cells were Min and fluorescence was cytoplasmic in all cases.

  • b Ring-like accumulations were detected at a low frequency in these filaments (see text).