TABLE 1.

Bacterial strains and plasmids used in this study

Strain, plasmid, or transposonRelevant characteristic(s)Source or reference
S. meliloti
    Rm1021SU47 str-21 Strr31
    Sm7019Rm1021 thuE::pThuE629, Φ(thuE′-lacZ) transcriptional fusion, Strr KmrThis work
    Sm7023Rm1021 thuA::Tn5-1062, Φ(thuA′-luxAB) transcriptional fusion, Strr KmrThis work
    Sm7025Rm9628 thuE::pThuE690 Strr Kmr TetrThis work
    Rm9628Rm1021 aglE192::Tn5 Strr Kmr47
E. coli
    DH5αsupE44 ΔlacU169 (φ80lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1
    S17-1/λpirRK2 tra regulon, λpir, host for pir-dependent plasmids42
    S327/λpirλpir, host for pir-dependent plasmids33
Plasmids
    pRL1062Bearing Tn5-1062, oriT(RK2) Kmr48
    pRK2013ColE1 Tra+ Kmr9
    pApa7023≈20-kb ApaI fragment from S. meliloti Sm7023 containing a thuA::Tn5-1062, KmrThis work
    pVIK112lacZY for transcriptional fusions, Kmr25
    pThuE629pVIK112, 629-bp thuE internal fragment, Φ(thuE′-lacZ) transcriptional fusion, KmrThis work
    pThuE690pSUP205, 690-bp thuE internal fragment, TetrThis work
    pSUP205mob λcos Cmr Tetr42
Transposon
    Tn5-1062Tn5 derivative, promoterless luxAB, oriV Kmr48