TABLE 1.

Bacterial strains or phage used in this study

Strain or phageRelevant genotype or descriptionReference or source
E. coli K-12 strains
    MG1655F λMichael Cashel
    CAG18642 zhf-331::Tn10; 57.5 min 30
    MC4100F Δ(argF-lac)U169 rpsL relA flhD deoC ptsF rbsR 11
    ML2a met gal hsdK R supE supF Δcya::Kanr 10
    SA2777aF rpsL relA Δcrp::camS. Garges and S. Adhya
    W3110F λmcrA mcrB IN(rrnD-rrnE)1Richard E. Wolf, Jr.
    TR1-5BW3414a csrA::Kanr 25
Clinical strains
    P5 C. freundii 14
    P18 E. coli 14
    P30 K. pneumoniae 14
    EF302 E. coli O157:H7 16
    ATCC 14028 S. enterica serovar Typhi- urium 2
Bacteriophage P1virStrictly lytic P1; forms clear plaques 30
  • a Mutant alleles Δcya::kanR, Δcrp::cam, and csrA::kanR were moved among strains by P1vir transduction or cotransduction. The strain prefix TR indicates the presence of the csrA::Kanr allele.