TABLE 2.

Fold induction of promoter activity in indicated medium conditiona

StrainMedium conditionResult for promoter fusion
mig-14::GFPpmrC::GFPpmrD::GFP
Mean peak fluorescence (aue)Fold inductionMean peak fluorescence (aue)Fold inductionMean peak fluorescence (aue)Fold induction
SL1344 (wild type)b10 μM MgCl239911.719919.936114.4
pH 5.8702.1343.4351.4
PB3339.812912.926610.6
Protamine2186.410710.72148.6
PG-11123.310710.71014.0
Triton X-100571.7131.3451.8
SDS561.6393.9532.1
phoP::Tn10c10 μM MgCl2611141
pH 5.8615530.75
PB264.31192.25
Protamine50.81151.25
PG-140.71141
Triton X-10061.01141
SDS61.01151.25
pmrA::Kand10 μM MgCl279820.51150911.6
pH 5.8671.711421
PB80520.6112124.8
Protamine2867.3112225.0
PG-11042.7111583.6
Triton X-100561.411581.3
SDS541.411741.7
  • a Mean peak fluorescence values are for the populations grown under the condition indicated. Fold inductions were calculated by dividing the mean peak fluorescence values for growth under the condition indicated by the mean peak fluorescence values for growth in 10 mM MgCl2 at pH 7.4 (values given in footnotes b, c, and d).

  • b Graphic representations of these results are shown in Fig. 1. Mean peak fluorescence values of the bacterial population grown in 10 mM MgCl2 at pH 7.4 are as follows: for mig-14::GFP, 34; for pmrC::GFP, 10; and for pmrD::GFP, 25.

  • c Graphic representations of these results are shown in Fig. 2. Mean peak fluorescence values of the bacterial population grown in 10 mM MgCl2 at pH 7.4 are as follows: for mig-14::GFP, 6; for pmrC::GFP, 1; and for pmrD::GFP, 4.

  • d Graphic representations of these results are shown in Fig. 3. Mean peak fluorescence values of the bacterial population grown in 10 mM MgCl2 at pH 7.4 are as follows: for mig-14::GFP, 39; for pmrC::GFP, 1; and for pmrD::GFP, 44.

  • e au, arbitrary units.