TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidCharacteristicsReference or source
B. subtilis
    CU1065W168 attSPβ trpC2Laboratory stock
    ZB307AW168 SPβc2Δ2::Tn917::pSK10Δ635
    HB1000ZB307A attSPβLaboratory stock
    HB1122ZB307A SPβc2Δ2::Tn917::Φ(mrgA-cat-lacZ)6
    HB0518ZB307A SPβc2Δ2::Tn917::Φ(katA-cat-lacZ)3
    HB8206ZB307A SPβc2Δ2::Tn917::Φ(zosA-cat-lacZ)A. Gaballa
    HB1602ZB307A SPβc2Δ2::Tn917::Φ(ahpC-cat-lacZ)1
    HB0539ZB307A SPβc2Δ2::Tn917::Φ(perR-cat-lacZ)This work
    HB6560ZB307A SPβc2Δ2::Tn917::Φ(fur-cat-lacZ)This work
    HB0568CU1065 SPβc2Δ2::Tn917::Φ(mrgA-cat-lacZ)This work
    HB0567CU1065 SPβc2Δ2::Tn917::Φ(katA-cat-lacZ)This work
    HB8108CU1065 SPβc2Δ2::Tn917::Φ(zosA-cat-lacZ)A. Gaballa
    HB2083CU1065 SPβc2Δ2::Tn917::Φ(ahpC-cat-lacZ)This work
    HB1041CU1065 hemA-lacZ6
    HB2062CU1065 SPβc2Δ2::Tn917::Φ(perR-cat-lacZ)This work
    HB2076CU1065 SPβc2Δ2::Tn917::Φ(fur-cat-lacZ)This work
    HB0509HB1000 perR::spc3
    HB2078CU1065 perR::kanThis work
    HB2116CU1065 perR::kan SPβc2Δ2::Tn917::Φ(mrgA-cat-lacZ)This work
    HB2117CU1065 perR::kan SPβc2Δ2::Tn917::Φ(katA-cat-lacZ)This work
    HB2118CU1065 perR::kan SPβc2Δ2::Tn917::Φ(zosA-cat-lacZ)This work
    HB2120CU1065 perR::kan SPβc2Δ2::Tn917::Φ(ahpC-cat-lacZ)This work
    HB2121CU1065 perR::kan hemA-lacZThis work
    HB2119CU1065 perR::kan SPβc2Δ2::Tn917::Φ(perR-cat-lacZ)This work
    HB2115CU1065 perR::kan SPβc2Δ2::Tn917::Φ(fur-cat-lacZ)This work
    HB2501CU1065 fur::kanThis work
    HB2082CU1065 fur::kan SPβc2Δ2::Tn917::Φ(mrgA-cat-lacZ)This work
    HB2110CU1065 fur::kan SPβc2Δ2::Tn917::Φ(katA-cat-lacZ)This work
    HB2111CU1065 fur::kan SPβc2Δ2::Tn917::Φ(zosA-cat-lacZ)This work
    HB2113CU1065 fur::kan SPβc2Δ2::Tn917::Φ(ahpC-cat-lacZ)This work
    HB2114CU1065 fur::kan hemA-lacZThis work
    HB2112CU1065 fur::kan SPβc2Δ2::Tn917::Φ(perR-cat-lacZ)This work
    HB2081CU1065 fur::kan SPβc2Δ2::Tn917::Φ(fur-cat-lacZ)This work
    HB7503CU1065 mntR::kan27
    HB2079CU1065 mntR::kan SPβc2Δ2::Tn917::Φ(fur-cat-lacZ)This work
    HB6507HB1000 ahpC::Tn10 (spc)1
    HB6566HB1000 ahpC::Tn10 (spc) SPβc2Δ2::Tn917::Φ(fur-cat-lacZ)This work
    HB1308Spontaneous katA mutant; cured of SPβ6
    HB6570HB1308 SPβc2Δ2::Tn917::Φ(fur-cat-lacZ)This work
E. coli DH5αLaboratory stock
Plasmids
    pBSKII+pBR322 repliconStratagene
    pJPM122cat-lacZ operon fusion vector for SPβ29
    pGEM-catpGEM-3zf(+)-cat-133
    pDG780pBSKII+ containing kanamycin resistance cassette13
    pHB6518pJPM122 with fur promoterThis work
    pSK1pGEM-cat with 542-bp fragment containing perR3
    pAFH3pBSKII+ with 542-bp fragment containing perRThis work
    pAFH5pJPM122 with perR promoterThis work
    pMF20pGEM-cat with 776-bp fragment containing perRThis work
    pMF21pGEM-cat with perR::kanThis work