TABLE 1.

Plasmids used in this study

PlasmidDescriptionReference
pUC19E. coli cloning vector, Apr, multiple cloning site36
pUCB2Shuttle vector for pUC19 and pUB110, Kmr PmrBrantl, unpublished data
pCATpUC19 with 1.4-kb CAT gene24
pAC6Vector for integration of transcriptional lacZ fusions42
pWH353E. coli-B. subtilis shuttle vector, Tet system, Kmr12
pWSR2pWH353 with bsrF gene under tet-inducible promoterThis study
pUCBSR2pUCB2 with bsrF gene under its own promoterThis study
pFRONT5pUC19 with 800 bp upstream of bsrFThis study
pBACK5pUC19 with 500 bp downstream of bsrFThis study
pCBACK5pBACK5 with CAT gene from pCATThis study
pINT5Vector for replacement of bsrF by CAT geneThis study
pACG6pAC6 with 415 bp upstream of bsrF transcription start siteThis study
pACG9pAC6 with 375 bp upstream of bsrF transcription start siteThis study
pACG10pAC6 with 302 bp upstream of bsrF transcription start siteThis study
pACG12pAC6 with 71 bp upstream of bsrF transcription start siteThis study
pACG13pAC6 with 89 bp upstream of bsrF transcription start siteThis study
pACG16pAC6 with 58 bp upstream of bsrF transcription start siteThis study
pACG17pAC6 with 48 bp upstream of bsrF transcription start siteThis study
pACG4pACG6 with 35 bp upstream of bsrF transcription start siteThis study
pACG11pACG6 with two point mutations in CodY binding siteThis study
pACG15pACG6 with one point mutation in CodY binding siteThis study
pACG18pACG6 with two point mutations in CodY binding siteThis study
pACG19pACG6 with two point mutations in CodY binding siteThis study