TABLE 1.

Oligonucleotide primers used in this study

PrimerSequenceaPositions of primers (GenBank accession no. AF498371)
TftDF1b5′-AGTACCTGGAGTC(G/C)CT(G/C)AACGAC-3′213-235
TftDR1b5′-CGG(C/G)GT(C/G)CCGTTGAATTCTCGAA-3′1543-1520
HQFb5′-AGGAGTTCATCCTGCT(G/C)(A/T)G-3′2623-2641
HQRb5′-CGCAC(G/C)CCGAACAC(A/T)GCGTC-3′3163-3144
TftDRcr5′-ATCGGCACCAACAAGATC-3′1136-1153
HQOFcr5′-AGGAATCGGATAGGCCTG-3′2999-2982
TftDRf5′-CTTGTTGGTGCCGATATG-3′1150-1133
TftDFcr5′-TGTTCCTTGTCGTAGTGG-3′429-412
TcpABC55′-GGAGTCCAGTGATGCAAG-3′2407-2424
TcpF65′-ATGACGTGCAGTACGAAG-3′2887-2904
TcpAiF5′-CCAAGAAGCACGACCTGA-3′621-638
TcpABC45′-CAGCCCGGCTGGTGTTCGT-3′1952-1969
TcpABC15′-AAGATAGCCGCCTTGCAG-3′2205-2188
TcpAF4-NdeI5′-AGACCTGCATATGATTCGCACTGG-3′184-207
TcpAR5-BamHI5′-GAGGCGGATCCACAGTCATCG-3′1754-1734
TcpF16-NdeI5′-GGAGTCCACATATGCAAGAGTATG-3′2407-2430
TcpR155′-CTTTCCGGTGCTAGAGGGTTGG-3′3258-3237
TcpF25′-TCCGCATGCTGACTATGC-3′1554-1571
  • a Boldface indicates changes from template DNA for introduction of restriction sites (underlined).

  • b Degenerate primer.