TABLE 2.

Phenotypic complementation of P. aeruginosa PAO1 vfr::Tc

Strain (plasmid)Vfr allelebPhenotypea
Twitching motilitycElastasedPyocyanine
LBAPIA
PAO1(pUCPSK)None25.0 ± 0.80.47 ± 0.130.34 ± 0.090.55 ± 0.11
PAO1 vfr::Tc(pUCPSK)None3.8 ± 0.40.04 ± 0.030.07 ± 0.034.13 ± 0.29
PAO1 vfr::Tc(pSB299.15A)Wild type24.5 ± 0.70.41 ± 0.130.17 ± 0.050.39 ± 0.04
PAO1 vfr::Tc(pSB299.17A)ΔEQERS5.3 ± 0.40.33 ± 0.080.24 ± 0.020.76 ± 0.10
  • a Mean results and standard deviations from three independent assays are given.

  • b Vfr allele carried on plasmid.

  • c Twitching zone diameter (millimeters) after 24 h of incubation.

  • d Elastolytic activity (optical density at 495 nm) of 16-h culture supernatant as determined by the Elastin Congo Red assay (33).

  • e Pyocyanin production (optical density at 520 nm) was assayed as described in Materials and Methods from 16-h PIA or LBA plate cultures.