TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant characteristic(s)aSource or reference
Bacterial strains
    C. acetobutylicum
        ATCC 824ATCCb
        SKO1ATCC 824 spo0A::MLSrThis study
    E. coli ER2275 recA lacZ mcrBC NEBc
Plasmids
    pAN1Cmr; carries the φ3TI gene 20
    pIMP1Apr 22
    pMSPOAApr MLSr; carries spo0AThis study
    pJC4MLSr Tcr 17
    pGEM-4ZAprPromegad
    pTHAADApr Cmr; carries aad 18
    pCLH2CmrThis study
    pSCLH2pCLH2 with 0.58-kb internal spo0A fragmentThis study
    pESCLH2pSCLH2 with MLSrThis study
    pETSPODerivative of pESCLH2This study
    pFNK6Apr MLSr; carries adc ctfA ctfB 21
    pHXS5Apr; carries orf1 aad ctfA ctfB 23
  • a Abbreviations: Cmr, chloramphenicol resistant; φ3TI, φ3TI methyltransferase; Apr, ampicillin resistant; Tcr, tetracycline resistant.

  • b ATCC, American Type Culture Collection, Manassas, Va.

  • c New England Biolabs.

  • d Promega, Madison, Wi.