TABLE 2.

Putative SlyA-regulated proteins of Salmonella serovar Typhimurium ATCC 14028s identified by MSa

Putative SlyA regulationSpotProteinGeneMol. mass (kDa)bpIb
Inductionc1GroEL (chaperone Hsp60) mopA 57.294.85
2OmpD (outer membrane porin) nmpC 39.68/37.63e4.66/4.57
3FliC (flagellin, flagellar filament structural protein) fliC 51.614.79
450S ribosomal subunit protein L7/L12 rplL 12.304.60
5PotD (spermidine/putrescine-binding periplasmic protein) potD 39.02/36.655.17/4.84
6 N-acetylneuraminate lyase (aldolase) subunit (NanA) nanA 32.465.56
7Putative ManNAc-6P epimerase nanE 24.035.07
8Glycerol dehydrogenase, NAD+ dependent (GldA) gldA 38.744.88
9Thiol peroxidase tpx 18.034.93
10Putative oxidoreductase ucpA 27.875.05
11Putative glutamate dehydrogenaseSTM179548.046.23
12Putative cytoplasmic protein yhcH 17.064.93
Repressiond13Periplasmic Cu,Zn-superoxide dismutase (SodCII) sodCII 17.74/15.816.03/5.69
14OsmY (hyperosmotically and carbon starvation-inducible periplasmic protein) osmY 21.45/18.555.78/5.14
15LuxS (quorum-sensing protein; autoinducer synthase) luxS 19.315.71
16Purine nucleoside phosphorylase (DeoD) deoD 25.985.56
17Putative oxidoreductase yohF 26.835.50
18Putative catalaseORF G57 (STM1731)31.854.62
19YgaU (putative LysM domain protein) ygaU 16.125.44
20Putative outer membrane or exported proteinSTM424246.98/44.454.69/4.56
21Periplasmic l-asparaginase II (AnsB) ansB 36.93/34.615.84/5.61
22YbdQ (function unknown) ybdQ 15.906.18
23YciF (putative cytoplasmic protein) yciF 18.655.24
  • a Cellular proteins from bacteria grown to the stationary phase were analyzed.

  • b Theoretical molecular (mol.) masses and pI values of the Salmonella serovar Typhimurium proteins were calculated from the corresponding amino acid sequences deposited in the NCBI protein database (http://www.ncbi.nlm.nih.gov/Entrez ) by using ExPASy proteomics tool Compute pI/Mw (http://www.expasy.ch ). Putative signal peptide cleavage sites in the precursors of the identified periplasmic and outer membrane (or exported) Salmonella serovar Typhimurium proteins were predicted as described by Nielsen et al. (33) with ExPASy proteomics tool SignalP.

  • c Proteins show higher expression levels in Salmonella serovar Typhimurium ATCC 14028s/pAL102 than in slyA mutant Salmonella serovar Typhimurium ATCC 14028slyA.

  • d Proteins show higher expression levels in Salmonella serovar Typhimurium ATCC 14028slyA than in Salmonella serovar Typhimurium ATCC 14028s/pAL102.

  • e Value for precursor form/value for predicted mature form.