TABLE 2.

ALA and tetracycline complementation of SL1344 wild-type and hemA26 mutant strains

StrainTetracycline (μg/ml)ALA (μg/ml)Generation timea (min)
SL1344029.4
SL13445033.3
SL134410033.9
SL1344/pTE367/(prfA)030.8
SL1344/Ptac-dorf1b032.1
LT2 hemA600378.4
LT2 hemA6010055.0
SL1344 hemA26070.8
SL1344 hemA2610031.7
SL1344 hemA265055.3
SL1344 hemA2610047.3
SL1344 hemA26/pTE367(prfA)072.1
SL1344 hemA26/pTE367(prfA)5048.6
SL1344 hemA26/pTE367(prfA)10044.8
SL1344 hemA26/Ptac-dorf1b067.9
SL1344 hemA26/Ptac-dorf1b5050.8
SL1344 hemA26/Ptac-dorf1b10047.7
  • a Cultures were grown in NB. Tetracycline (10 μg/ml) or ALA (50 or 100 μg/ml) was added where indicated.

  • b To induce expression of dorf1 from Ptac-dorf1 plasmid, the cells were diluted in NB medium supplemented with IPTG (0.1 μg/ml).