TABLE 2.

Multicopy dksA suppresses a ΔdksA deletion phenotype and some features of a ppGpp-dependent phenotypea

StrainAT sensitivityMinimum medium growthbGlycogen stain
MG1655 (wt)R+Brown
MG1655/pdksAR+Brown
CF1652 (ΔrelA)S+White
CF1652/pdksAS+Brown
CF1693 (ΔrelA ΔspoT)ScWhite
CF1693/pdksASBrown
PK2C1 (ΔdksA::kan)SdND
TE8114 (ΔdksA::tet)SdWhite
TE8114/pdksAR+Brown
TE8114/rpoB (T563P)R+Brown
  • a AT, aminotriazole; pdksA, pJK537; ND, not determined; S, sensitive; R, resistant; wt, wild type.

  • b Growth on M9 glucose minimal medium + uracil, +, growth; −, no growth.

  • c Requirements on dropout plates (uracil) + L, V, F, T, S, R, G, H, and probably I.

  • d Requirements on dropout plates (uracil) + L, V, G, F, T, (weak), and probably I.