TABLE 1.

Strains and plasmids used in this study

Strain or plasmidRelevant characteristicsaReference or source
Strains
    P. aeruginosa
        PAO1Wild type12, 27
        PAO1-SmSpontaneous Smr of PAO129
        PAO4455cbrB::ΩKm26
        PAO4479spuC::Ωsp/smThis study
        PAO4480spuC::Gm-GFP-FRTThis study
        PAO4486spuC::FRTThis study
        PAO4505aguBA::Gm25
        PAO5001aguA::Tc25
        PAO5003aguR::Tc25
        PAO5004PA0295::TcThis study
        PAO5005spuI::TcThis study
        PAO5006spuA::TcThis study
        PAO5007spuB::TcThis study
        PAO5008spuC::TcThis study
        PAO5009spuD::TcThis study
        PAO5010spuE::TcThis study
        PAO5011spuF::TcThis study
        PAO5012spuG::TcThis study
        PAO5013spuH::TcThis study
        PAO5100cbrAB::GmThis study
    E. coli
        DH5αF endA1 hsdR17 (rK mK+) supE44 thi-1 recA1 gyrA relA1 Δ(lacIZYA-argF)U169 deoR [Φ80dlacΔ(lacZ)M15]Bethesda Research Laboratories
        HB101supE44 hsdS20(rB-mB-) recA13 ara-14 proA2 lacY galK2 rpsL20 xyl-5 mtl-121
        S17-1pro thi hsdR Tpr Smr: chromosome::RP4-2 Rc::Mu-Km::Tn734
        SM10thi-1 thr leu tonA lacY supE recA::RP4-2-Tc::Mu (Kmr)14
Plasmids
    pEX18ApApr, ColE1 replicon, oriT sacB14
    pFLP2Apr, pRO1600 replicon, oriT sacB flp cI85714
    pGU2Apr, a cosmid clone carrying the agu locus25
    pGU101Apr, pQF52 derivative carrying the PspuI-lacZ fusionThis study
    pGU102Apr, pQF52 derivative carrying the PspuA-lacZ fusionThis study
    pHP45ΩSp/SmApr, pBR322 derivative, Ωsp/sm4
    pNIC6011Apr, pACYC177 and pVS1 replicons26
    pQF52Apr, lacZ translational fusion vector30
    pRK2013Kmr, tra (RK2)3
    pRTP1Apr, conjugation vector35
    pSP858Apr, pBR322 derivative carrying the Gm-GFP-FRT cassette14
    pYJ96Apr, pEX18Ap derivative carrying the 3.3-kb EcoR-XhoI spuC fragmentThis study
  • a Antibiotic resistance: Apr, ampicillin for E. coli and carbenicillin for P. aeruginosa; Gmr, gentamicin; Kmr, kanamycin; Smr, streptomycin; Spr, spectinomycin, Tcr, tetracycline.