TABLE 6.

Model results for reactions deleted in central metabolism under four different substrate availability conditionsa

Deleted reactionIn silico essentiality in medium
MinimalMinimal + glucoseMinimal + carbon sourcesMinimal + carbon sources + amino acids
ACEB
ACNB++++
AKO++++
ATOB++++
ENO+++
FBA+++
FRD++++
FUMC++++
G6PD1++++
G6PD2++++
GAP+++
GLTA++++
ICD++++
MAEB++++
PGI+++
PGK+++
PGL++++
PGM+++
PPA
PPSA+++
PRSA
RPE++
RPI++
SCOT++++
TKTA1++++
TKTA2++
TPI
  • a The composition of each of the four media is described in the Results section. Symbols: (+), ability to make all 47 biomass constituents in the absence of the indicated reaction; (−), inability to make the biomass constituents.