TABLE 2.

Features of bacteriophage φ3626 ORFs, gene products, functional assignments, and amino acid sequence homologies

ORFStart positionStop positiongPFunctional assignmentHomology(ies)b
kDapIa
15455719.27.2Terminase, small subunitφ105 (gp21), Sfi19 (gp161), bIL309 (gp38)
2550229567.75.9Terminase, large subunitφ105 (gp22 and gp23), φSLT (gp563), D3 (gp2)
32296356448.78.3Portal proteinφ105 (gp25), φSLT (gp412), D3 (gp4)
4355437156.49.7
53720432523.14.7Putative prohead proteaseφPV83 (gp41), φC31 (gp35), φPVL (gp5a)
6c4366563147.74.9Major capsid protein (Cps)φ105 (gp27), bIL285 (gp44), φadh (gp395)
34.34.7Processed form, cleaved between R114 and D115
75641591910.84.9φ105 (gp30)
85912623513.28.8
96236668517.08.7φ105 (gp32)
106687703714.04.4
11c7054765022.54.9Major tail protein (Tsh)φ105 (gp34)
127665797911.99.7
12.1d7665848532.24.3
13851511400103.810.0Tail tape measure protein (Tmp)φSLT (gp1374), TP901-1 (TMP), φPVL (gp15)
14114011210227.44.8PSA (gp13), bIL285 (gp53)
151210215053110.06.2PSA (gp14), bIL285 (gp54), φg1e (gp1608)
16c151061658455.15.5Minor structural proteinφ105 gp42
17165971690512.14.8
18169141766928.24.8bIL170 (gpl12, accession number AF009630 )
19176841806114.39.6Holinφ105 (gp45)
20181491919238.85.9EndolysinpIP404 (ORF10), C. perfringens (ORF10C), PSA (Ply), 12826 (Ply12)
21193821980415.94.8
22211192007041.39.9IntegraseT12 (Int), PSA (gp24), T270 (Int)
23215872113517.65.2PSA (gp26), φSLT (gp153), A118 (gp35)
24223302160827.45.1RepressorφSLT (gp101a), φgle (gp132), PBSX (Xre)
2522504227228.39.2CroA2 (Cro)
26227492364534.58.7φSLT (gp81a) φadh (Tec)
2723680238566.89.6Transcriptional regulatorφETA (gp13 [64]), φPV83 (gp12)
2823903240826.79.8
2924093242997.99.2
3024319245378.510.3
3124527248531.14.5
32248672541221.39.4Sigma factor C. acetobutylicum, B. subtilis, and C. perfringens
3325428255474.68.9
34255632631529.39.4φETA (gp22), φSLT (gp256), rlt (gp11 [63])
35263252760848.38.3HelicaseP1 (Ban), SPP1 (gp40), D3 (gp74)
3627625278257.94.4
3727825280619.34.9
3828058282707.96.4
39282632867915.44.9SSBeUI36 (gp141), bIL286 (SSB), A118 (SSB)
4028706289429.14.1
41290202929510.910.0
42293042957610.510.9SpoIIID C. acetobutylicum, B. subtilis, and other bacilli
43296383016821.18.7
44305153086513.59.5
45308583142421.44.7
46314283197321.410.2RecombinaseL2 (gp5 [44]), Wφ (Int [34]), 186 (Int [24])
47321533250013.49.5
4832533327187.44.6
49327353305812.69.9
50330393346716.69.8φSLT (gp104b), bIL286 (gp39), φadh (gp170)
  • a Predicted by computer analysis.

  • b For reference or accession numbers, see text.

  • c Experimentally shown.

  • d Translational frameshift might result in two C-terminally different products (see text).

  • e SSB, single-stranded-DNA binding protein.