TABLE 7.

In silico results compared to experimental results for the essentiality of 17 genes in the modela

Deleted reactionGene(s)Locus identification(s)In vitro essentialityIn silico essentiality in mediumAgree- ment
MinimalMinimal + glucoseMinimal + carbon sourcesMinimal + carbon sources + amino acids
ACPSacpSHP0808EYes
AROQaroQHP1038EYes
DCDdcdHP0372NE++++Yes
EDAedaHP1099E++++
EDDeddHP1100E++++
FBPfbpHP1385E+++
GLCDglcDHP0509EYes
GPT1/2/3gptHP0735E++++
MQOmqoHP0086E++
MURBmurBHP1418EYes
MURFmurFHP0740EYes
OORoorA, oorB, oorC, oorDHP0589, HP0590, HP0591, HP0588E++++
PORporA, porB, porC, porDHP1110, HP1111, HP1108, HP1109EYes
PYREpyrEHP1257EYes
SPEEspeEHP0832NE
TALtalHP1495NE++++Yes
TRXBtrxA, trxBHP0824, HP0825EYes
  • a All of the essential in silico predictions are shaded, with a − sign indicating the inability to produce the biomass demands. The in vitro essentiality data is taken from reference 10 (E, essential; NE, not essential).