TABLE 5.

Partial purification of butyryl-CoA dehydrogenase

Purification stepProtein amt (mg)Sp act (U/mg)Yield (%)Purification factor
Cell extract72NDaNAbNA
Soluble fraction45.412.191001
DEAE-Sepharose CL-6B (pH 8.0)5.1440.7237.83.34
Membrane fraction28.80.111001
Solubilized membrane protein14.160.532364.81
DEAE-Sepharose CL-6B (pH 8.0)0.76514.19342129
  • a ND, not determined.

  • b NA, not applicable.