TABLE 1.

Plasmids used in this study

PlasmidDescriptionReference
pBAD30Expression vector, Apr20
pBR322E. coli cloning vector, Apr Tcr7
pECA1039Cryptic plasmid from E. carotovora subsp. atroseptica strain SCRI 103914
pECA1039-Km5EZ::TN <NotI/KAN-3> mutant with mutation in pECA1039 toxI repeat 5This study
pECA1039-Km6EZ::TN <NotI/KAN-3> mutant with mutation in pECA1039 toxI repeat 4This study
pECA1039-Km8EZ::TN <NotI/KAN-3> mutant with mutation in pECA1039 toxI repeat 2This study
pECA1039-Km9EZ::TN <NotI/KAN-3> mutant with mutation in pECA1039 toxI repeat 5This study
pECA1039-Km12EZ::TN <NotI/KAN-3> control insertion in pECA103914
pECA1039-Km13EZ::TN <NotI/KAN-3> mutant with mutation in pECA1039 toxI repeat 2This study
pECA1039-Km15EZ::TN <NotI/KAN-3> mutant with mutation in pECA1039 toxI half-repeat 614
pECA1039-Km22EZ::TN <NotI/KAN-3> mutant with mutation in pECA1039 toxI repeat 2This study
pECA1039-Km23EZ::TN <NotI/KAN-3> mutant with mutation in pECA1039 toxN14
pMJ1toxI toxN with native promoter in pBR322, AprThis study
pMJ4toxI toxN-FLAG with native promoter in pBR322, AprThis study
pMJX1toxI toxN(Y10A) with native promoter in pBR322, AprThis study
pMJX2toxI toxN(K20A) with native promoter in pBR322, AprThis study
pMJX2-FtoxI toxN-FLAG(K20A) with native promoter in pBR322, AprThis study
pMJX3toxI toxN(K20E) with native promoter in pBR322, AprThis study
pMJX3-FtoxI toxN-FLAG(K20E) with native promoter in pBR322, AprThis study
pMJX5toxI toxN(G37A) with native promoter in pBR322, AprThis study
pMJX6toxI toxN(Y47A) with native promoter in pBR322, AprThis study
pMJX6-FtoxI toxN-FLAG(Y47A) with native promoter in pBR322, AprThis study
pMJX7toxI toxN(S53A) with native promoter in pBR322, AprThis study
pMJX8toxI toxN(S64A) with native promoter in pBR322, AprThis study
pMJX9toxI toxN(K87A) with native promoter in pBR322, AprThis study
pMJX10toxI toxN(K116A) with native promoter in pBR322, AprThis study
pMJX11toxI toxN(G142A) with native promoter in pBR322, AprThis study
pMJX12toxI toxN(C148A) with native promoter in pBR322, AprThis study
pRW50lacZ promoter probe vector, RK2 replicon, Tcr28
pTA46toxI toxN with native promoter in pBR322, Apr14
pTA47toxI toxN-FS with native promoter in pBR322, Apr14
pTA49ToxN expression vector, pBAD30 based, Apr14
pTA50ToxN-FS expression vector, pBAD30 based, Apr14
pTA53toxI toxN-FLAG(Y10A) with native promoter in pBR322, AprThis study
pTA55toxI toxN-FLAG(G37A) with native promoter in pBR322, AprThis study
pTA56toxI toxN-FLAG(S53A) with native promoter in pBR322, AprThis study
pTA57toxI toxN-FLAG(S64A) with native promoter in pBR322, AprThis study
pTA58toxI toxN-FLAG(K87A) with native promoter in pBR322, AprThis study
pTA59toxI toxN-FLAG(K116A) with native promoter in pBR322, AprThis study
pTA60toxI toxN-FLAG(G142A) with native promoter in pBR322, AprThis study
pTA61toxI toxN-FLAG(C148A) with native promoter in pBR322, AprThis study
pTA63toxI (3.5 repeats) toxN with native promoter in pBR322, AprThis study
pTA64toxI toxN(F35A) with native promoter in pBR322, AprThis study
pTA65toxI toxN(E154A) with native promoter in pBR322, AprThis study
pTA67toxI toxN-FLAG(F35A) with native promoter in pBR322, AprThis study
pTA68toxI toxN-FLAG(E154A) with native promoter in pBR322, AprThis study
pTA69toxI (4.5 repeats) toxN with native promoter in pBR322, AprThis study
pTA75toxI (2.5 repeats) toxN with native promoter in pBR322, AprThis study
pTA76toxI expression vector, pTA100 derivative, Smr Spr14
pTA100pQE-80L derivative, Smr Spr14
pTA104PtoxIN-lacZ fusion (transcriptional positions −200 to 3), pRW50 based, Tcr14
pTA106PtoxIN -toxI-lacZ fusion (transcriptional positions −200 to 253), pRW50 based, Tcr14
pTA119PtoxIN-toxI-lacZ fusion (transcriptional positions −200 to 228), pRW50 based, Tcr14
pTA120PtoxIN-toxIN-lacZ fusion (transcriptional positions −200 to 769), pRW50 based, TcrThis study
pTRB1ToxN-FLAG expression vector, pBAD30 based, Apr14
pTRB39ToxN-FLAG(Y10A) expression vector, pBAD30 based, AprThis study
pTRB40ToxN-FLAG(K20E) expression vector, pBAD30 based, AprThis study
pTRB42ToxN-FLAG(Y47A) expression vector, pBAD30 based, AprThis study
pTRB44ToxN-FLAG(K87A) expression vector, pBAD30 based, AprThis study
pTRB45ToxN-FLAG(C148A) expression vector, pBAD30 based, AprThis study
pTRB46ToxN-FLAG(E154A) expression vector, pBAD30 based, AprThis study
pTRB47ToxN-FLAG(K20A) expression vector, pBAD30 based, AprThis study
pTRB48ToxN-FLAG(F35A) expression vector, pBAD30 based, AprThis study
pTRB49ToxN-FLAG(S53A) expression vector, pBAD30 based, AprThis study
pTRB50ToxN-FLAG(S64A) expression vector, pBAD30 based, AprThis study
pTRB51ToxN-FLAG(K116A) expression vector, pBAD30 based, AprThis study
pTRB52ToxN-FLAG(G142A) expression vector, pBAD30 based, AprThis study
pTRB53ToxN-FLAG(G37A) expression vector, pBAD30 based, AprThis study