TABLE 2.

Reactivity of partially purified NADH:quinone oxidoreductase with various electron acceptors

Electron acceptor (concn [mM])Redox potential (mV)Extinction coefficient (mM−1 cm−1); wavelength (nm)TPZ concn (mM)Sp act of NADH:acceptor oxidoreductase (U/mg)
Duroquinone (0.2)+35dNAg052.3
0.120.1
Duroquinone (0.1)+35dNA015.7
0.14.02
1,4-Naphthoquinone (0.1)+64dNA080.4
0.137.4
Phenazine methosulfate (0.1)+80cNA0189.3
0.123.7
INT (0.4)-90a17.4; 465f068.9
NBT (0.4)-50a40.2; 605f00.47
TTC (0.4)-83,-240,-415b9.1; 546e041.2
Benzyl viologen (1)-360d8.65; 578d0456.6
Methyl viologen (1)-440d9.7; 578d023.2
  • a Karmakar et al. (14).

  • b Rich et al. (27).

  • c Denke et al. (6).

  • d Bergmeyer (4) and Friedrich and Schink (8).

  • e Li et al. (18).

  • f Altman (1).

  • g NA, not applicable.