TABLE 2.

Plasmid constructs used in this study

PlasmidDescriptionReference
pCJK47Conjugative donor plasmid, carries OriTpCF10, lacZ, and pheS, used in pLT06 construction20
pGB354Contains Catr, used in the construction of pLT063
pCASPERContains orfB, orfC, RepA(Ts), and orfD, used in pLT066
pAT28Broad-range shuttle vector, spectinomycin resistant39
pCPSC2Source of the CpsC promoter used in pLT0912
pKS05pCJK47 derivative containing CatrThis study
pLT06Deletion construct used for making mutantsThis study
pLT08pLT06 containing a 2.0-kb EcoRI/PstI fragment containing engineered cpsF deletionThis study
pLT09pAT28 containing a 398-bp EcoRI/BamHI fragment containing the native CpsC promoterThis study
pLT10pLT09 containing 851-bp SalI/SphI fragment containing cpsFThis study
pLT13pLT06 containing a 2.0-kb EcoRI/PstI fragment containing engineered cpsH deletionThis study
pLT14pLT09 containing a 447-bp BamHI/SphI fragment containing cpsHThis study
pLT16pLT06 containing a 2.0-kb BamHI/SmaI fragment containing engineered cpsD deletionThis study
pLT18pLT06 containing a 2.0-kb EcoRI/PstI fragment containing engineered cpsE deletionThis study
pLT22pLT06 containing a 2.0-kb EcoRI/PstI fragment containing engineered cpsC deletionThis study
pLT23pLT06 containing a 2.0-kb SmaI/SphI fragment containing engineered cpsG deletionThis study
pLT24pLT06 containing a 2.0-kb EcoRI/PstI fragment containing engineered cpsI deletionThis study
pLT25pLT09 containing a 1,418-bp BamHI/SphI fragment containing cpsDThis study
pLT32pLT09 containing a 2,562-bp BamHI/SalI fragment containing cpsEThis study
pLT33pLT09 containing a 2,555-bp SalI/SphI fragment containing cpsGThis study
pLT34pLT09 containing a 1,319-bp BamHI/SphI fragment containing cpsCThis study
pLT35pLT09 containing a 1,192-bp BamHI/SphI fragment containing cpsIThis study