TABLE 2.

Competition assays with Gifsy-2 deletion strains

Relevant genotypeaStrainMedian CIbNo. of micecPd
Δ(Gifsy-2)::KmJS2230.00684<0.0005
sodCI::aphJS1920.159<0.0005
Δ(attL-gftO)::KmJS2240.703NS
Δ(gftO-attR)::KmJS2250.008220.001
ΔailT::CmJS2270.944NS
Δ(G-2 B)::KmJS1590.159<0.0005
Δ(stfT-sseI)::KmJS2290.876NS
Δ(gtgC-attR)::KmJS2300.1830.001
ΔgtgC::KmJS2280.725NS
ΔgtgD::KmMA69380.756NS
ΔgtgF::KmMA70751.365NS
ΔgtgEF::KmJS2210.1610<0.0005
ΔgtgE::KmMA70730.1450.0006
  • a All strains competed against wild-type 14028 except for strain MA7073, which competed against strain MA6054 (ara-907 araD901::MudJ).

  • b CI was calculated according to the formula in Materials and Methods, with the mutant as strain A and the wild type as strain B.

  • c All assays were performed i.p. using BALB/c mice.

  • d Student's t test was used to compare output versus inoculum. NS, not significant.