TABLE 2.

Summary of sequential treatments for M. pneumoniae core enrichmenta

Treatment 1Treatment 2Treatment 3Presence of coresbPurityb
2% TX0.6 M KIYes+++
2% TX1.8 M KINoNA
2% TX-DNase I (50 μg/ml)2% TX-0.6 M KClYes
2% TX-0.6 M KClYes++
2% TX-0.6 M KCl2% TX-0.6 M KClYes+
2% TX-0.6 M KCl2% TX-0.6 M KCl2% TX-0.6 M KClYes+
2% TX-0.6 M KCl2% TX-0.6 M KCl2% TX-1.8 M KClYes+
2% TX-0.6 M KCl2% TX-1.8 M KClYes++
2% TX-0.6 M KCl0.6 M KI with 20% sucrYes+++
2% TX-0.6 M KCl0.6 M KI with 20% sucr1 M Gdn-HClYes++++
2% TX-0.6 M KCl0.6 M KI with 20% sucr1.2 M Gdn-HClPartial++
2% TX-0.6 M KCl0.6 M KI with 20% sucr1.5 M Gdn-HClNoNA
2% TX-0.6 M KCl0.6 M KI with 20% sucr2 M Gdn-HClNoNA
2% TX-0.6 M KCl2% Na deoxycholateNAYes+++
2% TX-0.6 M KCl2% Na deoxycholate1 M Gdn-HClPartial++
2% TX-0.6 M KCl2% Na deoxycholate1.5 M Gdn-HClPartial+
2% TX-0.6 M KCl2% Na deoxycholate2 M Gdn-HClNoNA
2% TX-0.6 M KCl2 M Gdn-HClNoNA
2% TX-0.6 M KCl2 M Gdn-HCl-20% sucrPartial+
2% TX-0.6 M KCl2 M KSCNNoNA
2% Na deoxycholate-1 M KClPartial+
1% CHAPS1 M Gdn-HClPartial+
500 mM Gdn-HClPartial+
1 M Gdn-HClNoNA
  • a Gdn-HCl, guanidine-HCl; sucr, sucrose; NA, not applicable.

  • b The presence/absence of cores and the purity thereof were determined by EM analyses following the indicated extraction treatments. Amounts of residual material associated with the cores following the last treatment are expressed as ++++, +++, ++, +, and −, from largest to smallest.