TABLE 1.

Strains and plasmids

Strain or plasmidRelevant genotype or descriptionPhenotypeaReference
Strains
    SAM238325-46
    NT372SAM23 yycF1TS9
    SAM1010SAM23 yycF1 Tn917lac::purA571TS, Emr9
    SAM1011SAM23 Tn917lac::purA571Emr9
    SAM1011s1SAM1011 ssa::pMP2376TcrThis work
    SAM1011s1r1SAM1011 ssa+EmrThis work
    SAM1287NT372 pMP532EmrThis work
Plasmids
    pMP16S. aureus-Escherichia coli shuttle vectorEmr Apr9
    pMP532pMP16(ssa nhaC)EmrThis work
    pMP1025pMP16(nhaC)EmrThis work
    pMP1029pMP16(ssa)EmrThis work
    pMP2376ssa disruption plasmidrep(Ts) Tcr AprThis work
  • a TS, temperature sensitive; Tcr and Apr, tetracycline and ampicillin resistance, respectively.