Genotypes and sources of gonococcal strains used in this study

StrainGenotype (reference)Sourcea
FA19Wild typeP. F. Sparling
WV30mtrR171FA6140 DNA × FA19
WV31mtrR171 mtrD::kanmtrD::kan PCR × WV30
WV32penA4FA6140 DNA × FA19
WV33penA4 mtrD::kanmtrD::kan PCR × WV32
WV34mtrR171 penA4FA6140 DNA × WV32
WV35mtrR171 mtrD::kan penA4mtrD::kan PCR × WV34
WV36mtrR171 penA4 pen BFA6140 DNA × WV34
WV37mtrR171 mtrD::kan penA4 penBmtrD::kan PCR × WV36
FA19ABpenA penBR. Nicholas
WV38mtrR171 penA penBFA6140 mtrR PCR × FA19AB
FA140mtrR140 penA2 penB2 (24)P. F. Sparling
WV24mtrR140 mtrD::kan penA2 penB2mtrD::kan PCR × FA140
FA6140mtrR171 penA4 penB ponA1P. F. Sparling
WV22mtrR171 mtrD:: kan penA4 penB ponA1mtrD:: kan PCR × FA6140
  • a Transformants are shown as donor DNA × recipient strain, produced as described in Materials and Methods. PCR products were gel purified prior to transformation. Gene descriptions: mtrR encodes a transcriptional repressor of MtrCDE expression; mtrD encodes the transporter component of the MtrCDE efflux system; penA is the structural gene for PBP-2; penB is an allele of porB, encoding the gonococcal porin; and ponA is the structural gene for PBP-1.