TABLE 2.

ChvI-regulated genes from microarray analysis with chvI(D52E)/chvI+ and chvI(K214T) strains

Avg SLR (SD)aGenebGene product and description
chvI(D52E)chvI(K214T)
1.60 (0.16)−0.85 (0.13)SMa1587EglC; endo-1,3-1,4-β-glycanase
0.75 (0.34)−1.09 (0.31)SMa1705Transcriptional regulator, MucR family
1.48 (0.38)−0.96 (0.30)SMb20072ABC transporter, periplasmic solute binding protein; induced by myo-inositol
0.88 (0.32)−0.63 (0.08)SMb20329ThuA; trehalose catabolism protein
0.90 (0.13)−0.89 (0.18)SMb20943ExoZ; acetyltransferase
1.17 (0.23)−1.33 (0.20)SMb20944ExoQ; Putative polysaccharide polymerase, similar to the Wzy protein
1.09 (0.18)−1.41 (0.18)SMb20945ExoF1; periplasmic protein involved in polysaccharide export
1.30 (0.20)−1.36 (0.21)SMb20946ExoY; exopolysaccharide production protein
0.70 (0.20)−0.79 (0.17)SMb20948ExoU; glucosyltransferase
0.80 (0.16)−1.58 (0.35)SMb20949ExoV; pyruvyltransferase
1.49 (0.27)−2.00 (0.29)SMb20954ExoH; succinyltransferase
1.36 (0.26)−1.52 (0.34)SMb20955ExoK; endo-β-1,3-1,4-glucanase
1.19 (0.13)−1.07 (0.16)SMb20956ExoL; putative glucosyltransferase
1.30 (0.14)−1.58 (0.13)SMb20957ExoA; glucosyltransferase
1.40 (0.13)−2.10 (0.41)SMb20958ExoM; glucosyltransferase
1.10 (0.16)−1.77 (0.40)SMb20959ExoO; glucosyltransferase
1.12 (0.22)−2.09 (0.17)SMb20960ExoN; UDPglucose pyrophosphorylase
1.22 (0.20)−1.80 (0.36)SMb20961ExoP; protein-tyrosine kinase
0.93 (0.58)−1.26 (0.27)SMb21026Hypothetical protein
1.39 (0.26)−1.26 (0.16)SMb21188Putative acyltransferase, possibly surface saccharide-specific acetyltransferase
1.41 (0.24)−1.46 (0.22)SMb21189Putative glycosyltransferase
0.79 (0.14)−1.23 (0.19)SMb21190Putative glycosyltransferase
1.32 (0.34)−2.86 (0.99)SMb21191MsbA2; polysaccharide-exporting ABC transporter, ATP binding and permease
2.44 (0.27)−3.22 (0.32)SMb21440Conserved hypothetical protein, signal peptide
0.54 (0.17)−0.70 (0.16)SMb21466PrsD; ABC transporter, ATP binding component, for low-molecular-wt succinoglycan biosynthesis
0.89 (0.12)−1.28 (0.25)SMb21467Hypothetical protein
1.23 (0.07)−1.27 (0.06)SMb21491Hypothetical exported protein
0.98 (0.18)−1.71 (0.14)SMb21690ExoW; glucosyltransferase
1.11 (0.20)−1.33 (0.15)SMc00062Hypothetical protein
1.11 (0.20)−0.93 (0.06)SMc00070Conserved hypothetical protein signal peptide
0.82 (0.16)−1.34 (0.24)SMc00084Hypothetical protein
0.77 (0.23)−1.80 (0.30)SMc00096Conserved hypothetical protein, signal peptide
0.52 (0.27)−1.02 (0.27)SMc00168SinI; N-acyl-l-homoserine lactone synthetase
0.82 (0.24)−0.88 (0.16)SMc00191Putative signal transduction histidine kinase, phosphotransfer (Hpt) region
0.81 (0.23)−2.18 (0.15)SMc00404Hypothetical protein
1.17 (0.15)−1.01 (0.10)SMc00432IolB; putative myo-inositol catabolism protein
1.10 (0.22)−0.95 (0.23)SMc00433IolE; putative myo-inositol catabolism protein
0.60 (0.10)−0.60 (0.05)SMc00604RopB1; outer membrane protein
1.35 (0.20)−1.45 (0.20)SMc01163Putative oxidoreductase
1.22 (0.18)−0.63 (0.31)SMc01165IolC; putative sugar kinase
1.26 (0.07)−0.72 (0.26)SMc01166IolD; putative malonic semialdehyde oxidative decarboxylase
0.85 (0.19)−0.71 (0.12)SMc01556Hypothetical protein signal peptide
2.08 (0.33)−2.76 (0.35)SMc01580Conserved hypothetical protein
1.89 (0.32)−2.49 (0.73)SMc01581Hypothetical transmembrane protein
0.96 (0.38)−1.15 (0.54)SMc01774Hypothetical protein signal peptide
1.72 (0.21)−2.41 (0.20)SMc01855Putative membrane-bound lytic transglycosylase, signal peptide
0.66 (0.11)−0.52 (0.14)SMc02242Hypothetical transmembrane protein
0.77 (0.09)−0.98 (0.15)SMc02317Hypothetical protein signal peptide
0.60 (0.10)−0.67 (0.09)SMc02552Hypothetical protein
1.26 (0.31)−0.63 (0.48)SMc02854Hypothetical protein signal peptide
0.53 (0.13)−0.61 (0.15)SMc02986Hypothetical transmembrane protein
1.13 (0.22)−1.32 (0.23)SMc03108Calcium binding protein
2.10 (0.50)−2.40 (0.88)SMc04236Putative glycine-rich cell wall structural transmembrane protein
1.55 (0.15)−1.22 (0.40)SMc04276Hypothetical protein signal peptide
0.72 (0.10)−0.69 (0.10)SMc04381OpgC; putative glucan succinyltransferase
−0.60 (0.24)0.71 (0.32)SMb20902cABC transporter periplasmic solute binding protein for mannose, l-xylose, glucose, and sorbose
−0.94 (0.44)0.53 (0.35)SMb21285cConserved hypothetical protein, transthyretin-like protein
−2.10 (0.28)1.04 (0.31)SMc00159cConserved hypothetical protein, signal peptide
−0.71 (0.11)0.69 (0.14)SMc00622cRnd1; RNase D
  • a Pairwise comparisons between the mutant and wild type yielded average SLR values for each gene. SLR is the log2 ratio of the change, so an SLR of 1 is equivalent to a twofold change. Genes listed here have an average SLR with an absolute value of 0.5 or greater for both the chvI(D52E)/chvI+ and chvI(K214T) strains compared to the wild type and an increase or decrease in expression levels in at least six of the nine pairwise comparisons with the wild type.

  • b The intergenic upstream regions for the genes with both the gene and gene product in boldface type were tested for binding to ChvI in gel mobility shift assays; those with only the gene product in boldface type appear in an operon whose upstream region was tested for binding to ChvI in gel mobility shift assays.

  • c Negatively regulated by ChvI. All other genes are positively regulated by ChvI.