TABLE 1.

Strains and plasmids

Straina or plasmidGenotype or relevant characteristicsReference or source
Strains
    Rm1021Derivative of RCR2011; Sm37
    Rm7095exoR95::Tn5; Sm Nm14
    Rm7096exoS96::Tn5; Sm Nm14
    EC69chvI(K214T) (integrated pDW181); Sm Hy53
    EC176Wild type (integrated pDW181); Sm Hy53
    EC220chvI(D52E)/chvI+ (integrated pEC177); Sm HyThis study
    EC407Wild type (integrated pEC406); Sm SpThis study
    EC409chvI(D52E)/chvI+ (integrated pEC405); Sm SpThis study
    EC412chvI(K214T) (integrated pEC406); Sm Sp9
Plasmids
    pDW33Identical to pVO155; Ap Hy13
    pDW181PhisB in pDW33, Ap Hy53
    pEC78PchvI and chvI in pCR2.1 TOPO; ApThis study
    pEC97PchvI and chvI(D52E) in pCR2.1 TOPO; ApThis study
    pEC177chvI(D52E) in pDW33; Ap HyThis study
    pEC340PexoY in pVO155; Ap Nm/KmThis study
    pEC405chvI(D52E) in pMB439; Ap SpThis study
    pEC406PhisB in pMB439; Ap Sp9
    pEC565SMc01855 internal fragment (bp 116-425) in pVO155; Ap Nm/KmThis study
    pEC571Psmb21188 in pVO155; Ap Nm/KmThis study
    pEC572Psmc00159 in pVO155; Ap Nm/KmThis study
    pEC573PropB1 in pVO155; Ap Nm/KmThis study
    pEC616Psmb21440 in pVO155; Ap Nm/KmThis study
    pEC617SMb21491 internal fragment (bp 110-442) in pVO155; Ap Nm/KmThis study
    pEC618Psmc01580 and SMc01580 in pVO155; Ap Nm/KmThis study
    pMB439pBluescript SK(−) derivative with Spr cassette; Ap Sp4
    pRF1206Position −359 through 5 bp of ropB1 ORF in pCR2.1 TOPO; Ap KmThis study
    pRF1211Position −143 through 11 bp after stop codon of SMc01580 in pCR2.1 TOPO; Ap KmThis study
    pRF1212Position −297 through start codon of SMb21440 in pCR2.1 TOPO; Ap KmThis study
    pRF1218Position −390 through −96 before start codon of SMc01580 in pCR2.1 TOPO; Ap KmThis study
    pRK600Conjugal transfer helper plasmid; Cm15
    pVO155Terminator and polylinker preceding uidA in pUC119 derivative; Ap Nm/Km38
  • a All strains are derived from Rm1021.