TABLE 1.

Strains, plasmids, and bacteriophages used in this study

Strain, plasmid, or phageRelevant characteristic(s)aSource
L. lactis
    W51Multiple plasmids, including pED1This study
    LM0230Plasmid-free host for 936- and c2-like phages 37
    MG1363Plasmid-free host for 936- and c2-like phages 27
    SMQ481Host for P335-like phages 7
E. coli
    DH5alphaCloning hostInvitrogen
    GM48 dam/dcm host
Plasmids
    pBSCloning vector for DNA sequencing, Apr, 2.9 kbStratagene
    pSA3 E. coli-L. lactis shuttle vector, Cmr Tcr Emr, 10.2 kb 13
    pMIG3 E. coli-L. lactis shuttle vector, Cmr, 5.5 kb 55
    pNZ123High-copy vector for L. lactis, Cmr, 2.8 kb 16
    pMG36cExpression vector for L. lactis, Cmr, 3.4 kb 53
    pED1Resident plasmid of W51, Abi+, 12.2 kbThis study
Bacteriophages
    p2936 species, small isometric headed, cos type 43
    p2k936 species, small isometric headed 8
    sk1936 species, small isometric headed 10
    jj50936 species, small isometric headed 33
    ul36P335 species, small isometric headed, pac type 41
    Q30P335 species, small isometric headed 39
    Q33P335 species, small isometric headed 39
    φ31P335 species, small isometric headed 1
    φ50P335 species, small isometric headed 1
    c2c2 species, prolate headed 49
    eb1c2 species, prolate headed 43
    ml3c2 species, prolate headed 43
  • a Apr, ampicillin resistance; Cmr, chloramphenicol resistance; Tcr, tetracycline resistance; Emr, erythromycin resistance; Abi+, active Abi mechanism.