TABLE 1.

Strains and plasmids used in this study

Strain or plasmidPropertiesSource or reference
Strains
    S. aureus
        NewmanWild type 8
        AS3Newman sae::Tn917 (Emr) 17
        ALC355Newman agr::tetM 52
        ALC637Newman sar::Tn917 LTV1 53
        Newman-29Newman Δsae::kanA 10
        DU5886Newman fnbA::Tcr fnbB::Emr 18
        mAH12Newman eap::Eryr 22
        MS64Newman sigB1(Am) Tcr 45
        UAMS-1Osteomyelitis isolate 11
        Cowan IWild typeATCC 12598
        COLWild type 9
        6850Wild type 3
        ISP479CDerivative of 8325-4 37
        ISP479R8325-4, rsbU repaired 49
        ISP479C-29ISP479C Δsae::kanA 10
    S. carnosus TM 300Wild type 44
Plasmids
    pCWSAE28 saePQRS ISP479C cloned into pCL84This work
    pCWSAE33 saePQRS Newman cloned into pCL84This work
    pCG7 sae P1 promoter-lacZ fusion, integration vector 10