TABLE 2.

PCR primers used in this study

Primer and purposeSequence
Reporter construction
    lacZF5′-CCCGGAATTCATGACCATGATTACGGATTCACTG
    lacZR5′-CTAGCTAGCTTATTTTTGACACCAGACCAACTGG
    0581PF5′-CTAGCTAGCTCGTGCGGCCCAGTGTG
    0581PR5′-CGGAATTCGGTATGTCCTCGTGAGATGTGG
    PzmpAF5′-CCCAAGCTTCTGGAGCGCTCGCATTCAC
    PzmpAR5′-CGCGGATCCGGGCAGCAGTCGAGACAGTTTCTT
    PlipF5′-CCCAAGCTTTCGATGCATGGGTGTCGGCG
    PlipR5′-CGCGGATCCGGAACGCATCGATTTGGCCAT
    PorbF5′-CCCAAGCTTTGTGATAGCCGAGCGAGCCG
    PorbR5′-CGCGGATCCGAAACTCGTCATGTGCGTGAAGTCCTT
Intrans expression
    cepI-F5′-CGCGGATCCATGCAGACCTTCGTTCACGAG
    cepI-R5′-CCCAAGCTTTCAGGCGGCGATAGCTTGC
    cepR-F5′-CGCGGATCCATGGAACTGCGCTGGCAG
    cepR-R5′-CCCAAGCTTTCAGGGTGCTTCGATGAGCC
RT-PCR analysis
    Bcas0207F5′-CGCTCGCGCATCTGTGGTTC
    Bcas0207R5′-CCGGCGAGTCGTGGCGTGTC
    zmpAF5′-GCGGCGGCGGCTCGGTCTAC
    zmpAR5′-CGGGATCGTTCGGGTTGTTCG
    lipAF5′-AACCGCGCCCGCCGACGACTAT
    lipAR5′-GCCCTGGCTGTGACCGACGAGATT
    lipBF5′-GCCGGCGTCGCGATGTGGAG
    lipBR5′-GCGCGGTCAGGCAATAGTCG
    orbIF5′-ACGCGTGCATTGCTGGGTCTGTTC
    orbIR5′-GCGCGGCCGTCGTATGCT