TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidPhenotype and/or characteristic(s)Source or reference
Strain
    B. cenocepacia
        J2315Wild-type strain, genomovar III of B. cepacia complexATCC
        d0581BDSF mutant strain derived from J2315 with Bcam0581 being deleted 4
        J2315(P0581-lacZ)Wild-type strain harboring the reporter construct pMLS7P0581-lacZThis study
        J2315(PzmpA-lacZ)Wild-type strain harboring the reporter construct pMLS7PzmpA-lacZThis study
        J2315(Plip-lacZ)Wild-type strain harboring the reporter construct pMLS7Plip-lacZThis study
        J2315(Porb-lacZ)Wild-type strain harboring the reporter construct pMLS7Porb-lacZThis study
        d0581(0581)Mutant strain d0581 harboring the expression construct pMLS7-0581 4
        d0581(P0581-lacZ)Mutant strain d0581 harboring the reporter construct pMLS7P0581-lacZThis study
        d0581(PzmpA-lacZ)Mutant strain d0581 harboring the reporter construct pMLS7PzmpA-lacZThis study
        d0581(Plip-lacZ)Mutant strain d0581 harboring the reporter construct pMLS7Plip-lacZThis study
        d0581(Porb-lacZ)Mutant strain d0581 harboring the reporter construct pMLS7Porb-lacZThis study
        d0581(cepI)Mutant strain d0581 harboring the expression construct pMLS7-cepIThis study
        d0581(cepR)Mutant strain d0581 harboring the expression construct pMLS7-cepRThis study
        cepR::Kmr cepR insertion mutant derived from strain H111 15
        cepR::Kmr (pBAH27) cepR insertion mutant harboring cepR in construct pBAH27 15
        d0581(0207)Mutant strain d0581 harboring the expression construct pMLS7-0207This study
    E. coli
        DH5α supE44 ΔlacU169 (φ80dlacZΔM15)hsdR17 recA1 endA1 gyrA96 thi-1 relA1 λpir Laboratory collection
        S17-1 pro res mutant mod +-integrated copy of RP4,mob + Laboratory collection
    X. campestris
        FE58Biosensor for DSF/BDSF 39
Plasmid
    pMLS7pBBR1 ori, P S7 promoter, Tpr mob + 23
    pMLS7-0581pMLS7 containing Bcam0581 4
    pMLS7P0581-lacZpMLS7 containing the Bcam0581 promoter-lacZ fusionThis study
    pMLS7PzmpA-lacZpMLS7 containing the zmpA promoter-lacZ fusionThis study
    pMLS7Plip-lacZpMLS7 containing the lipA and lipB promoter-lacZ fusionThis study
    pMLS7Porb-lacZpMLS7 containing the orb operon promoter-lacZ fusionThis study
    pMLS7-cepIpMLS7 containing cepI This study
    pMLS7-cepRpMLS7 containing cepR This study
    pMLS7-0207pMLS7 containing Bcas0207This study
    pBAH27pBBR1MCS-5 containing the cepR gene of B. cepacia strain H111 15