TABLE 1.

Expression ratios and statistical significance of acid-induced gene comparisons

b no.GeneGene productFunctional groupSize (amino acids)Log10 expression ratioaPb
pH 5.5pH 4.5pH 5.5pH 4.5
Expt 1Expt 2Expt 1Expt 2
b1661cfaCyclopropane fatty acyl phospholipid synthaseCell envelope3820.3870.094e0.4709.47E-068.09E-022.35E-08
b1795yeaQPutative transglycosylase-associated membrane proteinCell envelope820.3660.2540.254e3.82E-047.18E-033.60E-03
b2215ompCOuter membrane protein 1b (lb;c)Cell envelope3670.5830.4050.410e1.27E-041.38E-043.71E-04
b2253yfbEPutative aminotransferase; modification of lipid A with aminoarabinoseCell envelope3790.7040.3520.300e3.57E-045.24E-041.90E-03
b2254yfbFPutative glycosyl transferase; LPS biosynthesisCell envelope2540.3710.2630.180e2.83E-042.09E-051.06E-03
b3506slpcOuter membrane lipoprotein; induced by stationary phase and starvationCell envelope1880.7100.4620.9768.54E-056.01E-056.88E-10
b3509hdeBcPutative periplasmic protein; unknown functionCell envelope1081.2870.6251.1131.60E-092.38E-044.70E-06
b3511hdeDcPutative membrane protein; unknown functionCell envelope1900.9420.3420.5844.50E-068.59E-056.93E-07
b4376osmYPeriplasmic protein; hyperosmotically inducibleCell envelope2010.4140.2590.107e6.33E-033.32E-029.68E-02
b1000cbpACurved-DNA binding protein; functions closely related to DnaJChaperones3060.3520.244e0.4732.07E-031.70E-031.92E-05
b3510hdeAcPeriplasmic chaperone; acid resistance phenotypeChaperones1101.3090.6771.1871.18E-071.61E-031.57E-05
b0329yahOORF, hypothetical protein; induced in stationary phaseHypothetical910.4980.2850.383e4.37E-051.37E-021.13E-03
b1003yccJcORF, hypothetical proteinHypothetical750.5550.2740.4613.56E-087.87E-032.17E-05
b1597asrcAcid shock RNA; ORF, hypothetical proteinHypothetical1021.8891.8282.3632.68E-089.31E-153.86E-06
b1724ydiZORF, hypothetical proteinHypothetical960.4480.152e0.5146.61E-052.84E-017.78E-06
b1836yebVORF, hypothetical proteinHypothetical780.4560.204e0.8338.38E-047.82E-023.10E-07
b3491yhiMcORF, hypothetical proteinHypothetical3640.9560.5591.0307.35E-069.45E-041.33E-07
b1004wrbAPutative flavodoxin proteinMetabolism1980.3440.193e0.4634.18E-047.97E-028.23E-06
b2885ygfRcPutative xanthine permeaseMetabolism4550.4960.4210.7221.47E-024.38E-038.54E-04
b4186yjfCPutative synthetase/amidaseMetabolism3870.4550.151e0.5451.84E-047.28E-027.15E-07
b0485ybaScPutative glutaminaseMetabolism3100.5200.3550.7551.97E-042.31E-062.72E-10
b0486ybaTdPutative amino acid/amine transporterMetabolism4300.186a0.147e0.404e1.11E-025.55E-031.05E-07
b0897ycaCPutative isochorismataseMetabolism2080.5650.239e0.8282.55E-061.11E-021.33E-07
b1493gadBcGlutamate decarboxylase isozymeMetabolism4660.8840.6371.1353.51E-053.01E-035.27E-10
b3517gadAcGlutamate decarboxylase isozymeMetabolism4660.9080.8051.4257.36E-043.90E-041.12E-10
b3507yhiFdPutative luxR transcriptional regulatorRegulatory1760.7460.079e0.379e3.05E-083.52E-014.66E-07
b3512yhiEcPutative luxR transcriptional regulatorRegulatory1751.1140.6021.2422.40E-054.54E-051.05E-07
b3515yhiWdPutative ARAC-type (family 2) regulatory proteinRegulatory2420.6100.071e0.073e6.24E-062.54E-021.10E-01
b3516yhiXcPutative ARAC-type (family 2) regulatory proteinRegulatory2740.8040.4300.8366.71E-076.29E-045.69E-06
b3555yiaGcPutative transcriptional regulator; helix-turn-helix motifRegulatory960.4980.2640.5861.07E-038.75E-039.34E-05
b0812dpscDNA binding protein; global regulator, starvation conditionsRegulatory1670.6750.4010.7802.97E-052.47E-041.79E-06
  • a Log induction ratio of experimental pH versus pH 7.4.

  • b P value calculated by application of Student’s t test for replicates of natural log-transformed, normalized spot intensities.

  • c Significantly induced in all three experiments.

  • d Significantly induced in only one experiment.

  • e Induction ratio not significantly different.