TABLE 4.

Pyocyanin production, nematode killing, and PQS production by P. aeruginosa mutants

StrainMutant genePyocyanina% Nematode killingaPQS
MPAO1(1.00)98 (2)+++
phz1 cluster
    MP601phzM0.01 (0.00)100 (0)+++
    MP705phzA10.04 (0.01)99 (1)+++
    MP706phzE10.17 (0.02)99 (1)+++
    MP702phzS0.02 (0.00)78 (11)+++
phnAB region
    MP703PA0998 (pqsC)0.04 (0.00)36 (8)
    MP704PA0999 (pqsD)0.02 (0.01)14 (2)
    MP605PA1000 (pqsE)0.01 (0.01)25 (8)+++
    MP710phnA0.04 (0.02)37 (8)
    MP551PA1003 (pqsR)0.01 (0.00)12 (10)
PA2587 region (MP562)PA2587 (pqsH)0.02 (0.01)9 (4)
Known regulators
    MP701lasR0.01 (0.00)2 (1)+/−
    MP607rhll/R0.02 (0.00)11 (8)+++
    MP502gacS0.10 (0.01)50 (5)+++
Putative regulators
    MP501PA39460.31 (0.02)22 (7)+++
    MP552PA47250.19 (0.04)19 (8)+++
    MP610PA48860.29 (0.03)40 (6)+++
    MP611np200.00 (0.00)4 (3)+/−
Miscellaneous
    MP613PA04060.07 (0.00)48 (17)+
    MP504PA07450.03 (0.01)50 (13)+
    MP615PA3031/PA30320.03 (0.01)1 (1)+
  • a Values listed are averages from three separate assays. Pyocyanin recoveries relative to the parent strain (MPAO1) are presented. Numbers in parentheses indicate standard errors of the mean.