TABLE 1.

Mutants defective in pyocyanin productiona

StrainParentInsert siteGeneDescriptionSource or reference
phz1 region
    MP601MPAO14,712,812phzMPyocyanin biosynthesisThis study
    MP651PAO1seq4,714,312phzA1Core phenazine biosynthetic operonThis study
    *MP705MPAO14,714,312phzA1Core phenazine biosynthetic operonThis studyb
    MP602MPAO14,715,531phzC1Core phenazine biosynthetic operonThis study
    MP652PAO1seq4,718,219phzE1Core phenazine biosynthetic operonThis study
    *MP706MPAO14,718,219phzE1Core phenazine biosynthetic operonThis studyb
    MP653PAO1seq4,720,699phzSPyocyanin biosynthesisThis study
    *MP702MPAO14,720,699phzSPyocyanin biosynthesisThis studyb
phnAB region
    MP654PA01seq1,081,164PA0998 (pqsC)β-Ketoacyl-acyl protein synthaseThis study
    *MP703MPAO11,081,164PA0998 (pqsC)β-Ketoacyl-acyl protein synthaseThis studyb
    MP603MPAO11,081,418PA0998 (pqsC)β-Ketoacyl-acyl protein synthaseThis study
    MP604MPAO11,081,470PA0998 (pqsC)β-Ketoacyl-acyl protein synthaseThis study
    MP655PAO1seq1,082,732PA0999 (pqsD)β-Ketoacyl-acyl protein synthaseThis study
    *MP704MPAO11,082,732PA0999 (pqsD)β-Ketoacyl-acyl protein synthaseThis studyb
    MP605MPAO11,082,972PA1000 (pqsE)HypotheticalThis study
    MP710MPAO1NDcphnAAnthranilate synthase.This study
    MP551MPAO11,086,674PA1003 (pqsR)lysR-like transcriptional regulator14
PA2587 region
    MP562MPAO12,927,500PA2587 (pqsH)FAD-dependent monooxygenase14
    MP606MPAO12,928,247PA2587/PA2588Between genesThis study
Known regulators
    MP701MPAO1NDlasRlasR quorum-sensing regulator15; this study
    MP607MPAO13,889,853rhll/Rrhl quorum-sensing regulatory locusThis study
    MP608MPAO11,013,341gacSGlobal regulatorThis study
    MP502MPAO11,015,249gacSGlobal regulator14
Putative regulators
    MP501MPAO14,423,808PA3946Similar to B. pertussis virulence gene regulator bvgS14
    MP609MPAO15,302,623dksARepressor of rhllThis study
    MP553MPAO15,304,386PA4725Putative two-component sensor kinase14
    MP552MPAO15,304,811PA4725Putative two-component sensor kinase14
    MP656PAO1seq5,306,302PA4725Putative two-component sensor kinaseThis study
    MP610MPAO15,482,018PA4886Putative two-component sensor kinase.This study
    MP611MPAO16,192,554np20Similar to E. coli zinc uptake regulator ZurThis study
Miscellaneous
    MP612MPAO1447,569PA0406Hypothetical proteinThis study
    MP613MPAO1448,033PA0406Hypothetical proteinThis study
    MP614MPAO1812,055PA0744Probable enoyl-CoA hydratase/isomeraseThis study
    MP504MPAO1812,969PA0745Probable enoyl-CoA hydratase/isomerase14
    MP615MPAO13,395,202PA3031/PA3032Between genesThis study
  • a All mutants correspond to insertions of ISphoA/hah-Tc except the phnA and lasR mutants, which were constructed by in vitro methods.

  • b Strain was derived by transferring into the chromosome of MPAO1 the insertion allele from the strain listed immediately above in the list.

  • c ND, not determined