TABLE 1.

Mutants defective in pyocyanin productiona

StrainParentInsert siteGeneDescriptionSource or reference
phz1 region
    MP601MPAO14,712,812 phzM Pyocyanin biosynthesisThis study
    MP651PAO1seq4,714,312 phzA1 Core phenazine biosynthetic operonThis study
    *MP705MPAO14,714,312 phzA1 Core phenazine biosynthetic operonThis studyb
    MP602MPAO14,715,531 phzC1 Core phenazine biosynthetic operonThis study
    MP652PAO1seq4,718,219 phzE1 Core phenazine biosynthetic operonThis study
    *MP706MPAO14,718,219 phzE1 Core phenazine biosynthetic operonThis studyb
    MP653PAO1seq4,720,699 phzS Pyocyanin biosynthesisThis study
    *MP702MPAO14,720,699 phzS Pyocyanin biosynthesisThis studyb
phnAB region
    MP654PA01seq1,081,164PA0998 (pqsC)β-Ketoacyl-acyl protein synthaseThis study
    *MP703MPAO11,081,164PA0998 (pqsC)β-Ketoacyl-acyl protein synthaseThis studyb
    MP603MPAO11,081,418PA0998 (pqsC)β-Ketoacyl-acyl protein synthaseThis study
    MP604MPAO11,081,470PA0998 (pqsC)β-Ketoacyl-acyl protein synthaseThis study
    MP655PAO1seq1,082,732PA0999 (pqsD)β-Ketoacyl-acyl protein synthaseThis study
    *MP704MPAO11,082,732PA0999 (pqsD)β-Ketoacyl-acyl protein synthaseThis studyb
    MP605MPAO11,082,972PA1000 (pqsE)HypotheticalThis study
    MP710MPAO1NDc phnA Anthranilate synthase.This study
    MP551MPAO11,086,674PA1003 (pqsR) lysR-like transcriptional regulator 14
PA2587 region
    MP562MPAO12,927,500PA2587 (pqsH)FAD-dependent monooxygenase 14
    MP606MPAO12,928,247PA2587/PA2588Between genesThis study
Known regulators
    MP701MPAO1ND lasR lasR quorum-sensing regulator 15; this study
    MP607MPAO13,889,853 rhll/R rhl quorum-sensing regulatory locusThis study
    MP608MPAO11,013,341 gacS Global regulatorThis study
    MP502MPAO11,015,249 gacS Global regulator 14
Putative regulators
    MP501MPAO14,423,808PA3946Similar to B. pertussis virulence gene regulator bvgS 14
    MP609MPAO15,302,623 dksA Repressor of rhllThis study
    MP553MPAO15,304,386PA4725Putative two-component sensor kinase 14
    MP552MPAO15,304,811PA4725Putative two-component sensor kinase 14
    MP656PAO1seq5,306,302PA4725Putative two-component sensor kinaseThis study
    MP610MPAO15,482,018PA4886Putative two-component sensor kinase.This study
    MP611MPAO16,192,554np20Similar to E. coli zinc uptake regulator ZurThis study
Miscellaneous
    MP612MPAO1447,569PA0406Hypothetical proteinThis study
    MP613MPAO1448,033PA0406Hypothetical proteinThis study
    MP614MPAO1812,055PA0744Probable enoyl-CoA hydratase/isomeraseThis study
    MP504MPAO1812,969PA0745Probable enoyl-CoA hydratase/isomerase 14
    MP615MPAO13,395,202PA3031/PA3032Between genesThis study
  • a All mutants correspond to insertions of ISphoA/hah-Tc except the phnA and lasR mutants, which were constructed by in vitro methods.

  • b Strain was derived by transferring into the chromosome of MPAO1 the insertion allele from the strain listed immediately above in the list.

  • c ND, not determined