TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidGenotype or constructionSource or reference
E. coli
    TG1supE hsdΔ5 thi Δ(lac-proAB) F′ (traD36 proAB+ laclq lacZΔM15)15
    CC118 λpirΔ(ara-leu) araD ΔlacX74 galE galK phoA20 thi-1 rpsE rpoB argE(Am) recA1 λpir phage lysogen29
P. putida
    PaW85Tn46525
    PKS54Tn4652 rpoS::km47
    PaW85 mutY::tetTn4652 mutY::tetThis work
Plasmids
    pEST1414Plasmid pAYC32 carrying promoterless pheBA operon32
    pBluescript SK(+)Cloning vector (Apr)Stratagene
    pBR322Cloning vector (Apr Tetr)8
    pBlscrMutYpBluescript SK(+) containing PCR-amplified P. putida mutY gene cloned into EcoRV- and SmaI-cleaved vectorThis work
    pBLscrMutY-Tetrtet gene from pBR322 inserted into EcoRI-Eco47III-cleaved mutY gene in pBLscrMutYThis work
    pGP704 LDelivery plasmid for homologous recombination50
    pGPMutY-TetrmutY::tet-sequence-containing SacI-KpnI fragment from pBlscrMutY-Tet cloned into pGP704LThis work
    pRK2013Helper plasmid for conjugal transfer of pGP704L16