TABLE 1.

Transcripts induced in UV-B and intense white light irradiation experimentsa

Category and functionGeneORFTreated/control ratiob
UV low, 20 minUV low, 2 hUV high, 2 hWhite light, 2 h
Amino acid biosynthesis
    Acetolactate synthaseilvBsll19811.38 ± 0.221.35 ± 0.262.00 ± 0.341.93 ± 0.28
Cofactor biosynthesis
    Geranylgeranyl pyrophosphate synthasecrtEslr06111.8 ± 0.551.82 ± 0.175.11 ± 1.081.57 ± 0.27
    Phytoene desaturasecrtPslr12541.96 ± 0.952.68 ± 0.874.33 ± 2.524.67 ± 1.44
    ζ-Carotene desaturasecrtQslr09401.42 ± 0.511.82 ± 0.332.29 ± 0.762.51 ± 0.63
    FerrochelatasehemHslr08391.27 ± 0.361.47 ± 0.292.2 ± 0.811.52 ± 0.18
    UbiH proteinubiHslr13001.63 ± 0.241.7 ± 0.183.45 ± 1.082.03 ± 0.46
    GTP cyclohydrolase IIribAsll18941.51 ± 0.261.69 ± 0.333.09 ± 1.491.36 ± 0.32
    Glutaredoxin 3grxCssr20611.1 ± 0.231.35 ± 0.131.98 ± 0.421.32 ± 0.26
    Thioredoxin MtrxMslr02331.06 ± 0.211.14 ± 0.492.01 ± 0.521.23 ± 0.19
    Glutathione peroxidaseslr11711.48 ± 0.551.61 ± 0.533.69 ± 1.631.78 ± 0.77
    Glutathione peroxidaseslr19922.55 ± 0.811.98 ± 0.353.03 ± 0.643.9 ± 1.17
Cell envelope
    Rare lipoprotein ArlpAslr04230.9 ± 0.160.84 ± 0.290.72 ± 0.343.79 ± 0.92
    Penicillin-binding protein 4pbpsll11672.16 ± 0.341.26 ± 0.392.08 ± 0.21.01 ± 0.27
Cellular processes
    Cell division protein FtsHftsHslr13902.22 ± 0.541.75 ± 0.343.39 ± 1.291.89 ± 0.68
    Cell division protein FtsHftsHslr02283.26 ± 0.972.52 ± 0.385.3 ± 2.691.84 ± 0.65
    Cell division protein FtsHftsHslr16043.63 ± 1.122.74 ± 0.526.07 ± 3.032.17 ± 0.8
    Cell division protein FtsHftsHsll14631.25 ± 0.101.33 ± 0.232.03 ± 0.41.45 ± 0.24
    MAFmafsll09051.34 ± 0.31.45 ± 0.432.09 ± 0.551.05 ± 0.05
    DnaJdnaJsll08971.72 ± 0.251.49 ± 0.233.22 ± 1.031.63 ± 0.15
    DnaJdnaJslr00932.13 ± 0.351.31 ± 0.42.35 ± 0.641.53 ± 0.22
    DnaK proteindnaKsll19322.08 ± 0.371.54 ± 0.252.19 ± 0.811.63 ± 0.31
    DnaK proteindnaKsll01703.48 ± 0.641.72 ± 0.343.89 ± 1.291.94 ± 0.52
    60-kDa chaperonin 1groELslr20762.25 ± 0.871.6 ± 0.631.82 ± 1.094.33 ± 2.42
    60-kDa chaperonin 2groEL-2sll04162.76 ± 1.011.87 ± 0.643.74 ± 2.083.2 ± 1.26
    10-kDa chaperoningroESslr20752.27 ± 0.551.53 ± 0.473.23 ± 1.643.94 ± 1.8
    Heat shock proteinhtpGsll04303.73 ± 1.421.93 ± 0.746.23 ± 2.891.85 ± 0.8
    Small heat shock proteinhsp17sll15147.8 ± 4.183.52 ± 3.4921.49 ± 11.892.89 ± 0.81
    Superoxide dismutasesodBslr15162.59 ± 0.521.31 ± 0.426.14 ± 3.381.81 ± 0.5
Energy metabolism
    P protein of glycine cleavage complexgcvPslr02931.51 ± 0.321.34 ± 0.122.2 ± 0.342.11 ± 0.25
    Glucose dehydrogenase BgdhBslr16081.66 ± 0.361.65 ± 0.112.89 ± 0.771.49 ± 0.27
    Mannose-1-phosphate guanyltransferasesll15581.46 ± 0.291.72 ± 0.373.51 ± 1.031.42 ± 0.18
    3-Ketoacyl-acyl carrier protein reductasefabGsll03302.47 ± 1.11.31 ± 0.254.48 ± 1.845.15 ± 2.22
    Squalene-hopene-cyclaseshcslr20891.72 ± 0.41.5 ± 0.241.95 ± 0.662.50 ± 0.88
Photosynthesis and respiration
    Ribulose bisphosphate carboxylase large subunitrbcLslr00090.69 ± 0.260.6 ± 0.270.26 ± 0.152.1 ± 1.22
    Ribulose bisphosphate carboxylase small subunitrbcSslr00120.65 ± 0.220.69 ± 0.340.2 ± 0.112 ± 0.93
    ChaperonerbcXslr00110.7 ± 0.310.79 ± 0.420.2 ± 0.132.12 ± 1.11
    Bicarbonate transportercmpAslr00401.59 ± 0.701.31 ± 1.011.93 ± 1.487.74 ± 4.48
    Bicarbonate transportercmpBslr00411.18 ± 0.450.95 ± 0.211.54 ± 1.144.79 ± 1.41
    Bicarbonate transportercmpCslr00431.34 ± 0.281.34 ± 0.751.44 ± 0.972.88 ± 1.19
    CO2-concentrating mechanism protein CcmKccmKsll10280.7 ± 0.250.74 ± 0.230.36 ± 0.12.47 ± 0.9
    CO2-concentrating mechanism protein CcmKccmKsll10290.71 ± 0.20.71 ± 0.220.38 ± 0.052.52 ± 0.75
    CO2-concentrating mechanism protein CcmLccmLsll10300.8 ± 0.210.71 ± 0.190.51 ± 0.242.56 ± 0.19
    CO2-concentrating mechanism protein CcmMccmMsll10310.81 ± 0.20.73 ± 0.120.34 ± 0.022.67 ± 0.42
    NADH dehydrogenasendhBslr17431.34 ± 0.241.41 ± 0.222.17 ± 0.542.13 ± 0.67
    NADH dehydrogenase subunit 4ndhD3sll17330.81 ± 0.320.68 ± 0.270.43 ± 0.075.88 ± 3.02
    NADH dehydrogenase subunit 4ndhDslr20070.89 ± 0.161.00 ± 0.330.95 ± 0.483.49 ± 0.94
    NADH dehydrogenase subunit 4ndhD2slr12911.81 ± 0.471.62 ± 0.212.37 ± 1.006.9 ± 3.65
    NADH dehydrogenase subunit 5ndhFsll17320.74 ± 0.290.66 ± 0.170.57 ± 0.124.89 ± 2.23
    Photosystem II D1 proteinpsbA2slr13114.41 ± 1.544.04 ± 1.275.89 ± 3.822.57 ± 1.82
    Photosystem II D1 proteinpsbA3sll18674.04 ± 0.673.85 ± 0.575.46 ± 3.022.26 ± 0.98
    Phycobilisome degradation protein NblAnblAssl04521.97 ± 0.392.13 ± 1.114.2 ± 1.771.2 ± 0.51
    Phycobilisome degradation protein NblAnblAssl04531.5 ± 0.241.36 ± 0.272.26 ± 1.181.14 ± 0.29
    Phycobilisome degradation protein NblBnblBslr16873.9 ± 0.612.73 ± 0.7919.91 ± 6.331.72 ± 0.41
    Cytochrome CytMcytMsll12451.35 ± 0.211.36 ± 0.141.97 ± 0.681.21 ± 0.21
    PlastocyaninpetEsll01990.95 ± 0.121.03 ± 0.181.97 ± 0.570.89 ± 0.1
    Photosystem II reaction center W proteinpsbWslr17391.29 ± 0.370.92 ± 0.872.2 ± 0.661.52 ± 0.36
Regulatory functions
    Transcription regulatory proteinlumQslr12131.18 ± 0.241.11 ± 0.370.9 ± 0.733.16 ± 0.6
    Transcription regulator Fur familyslr17381.63 ± 0.541.5 ± 0.262.92 ± 0.971.55 ± 0.32
    Two-component sensor histidine kinaseslr12853.13 ± 0.651.38 ± 0.744.08 ± 1.691.25 ± 0.19/PICK>
Transcription
    RNase Emeslr11292.18 ± 0.572.15 ± 0.572.16 ± 1.011.27 ± 0.44
    RNA polymerase sigma factorrpoDsll20122.45 ± 1.033.03 ± 0.6610.68 ± 5.072.21 ± 1.06
    RNA polymerase sigma factorrpoDsll01841.36 ± 0.411.17 ± 0.222.49 ± 0.761.74 ± 0.25
    RNA polymerase sigma factorrpoDsll03064.21 ± 0.61.68 ± 1.085.4 ± 0.592.8 ± 0.32
Translation
    ClpBclpBslr16416.57 ± 2.151.79 ± 0.649.01 ± 2.761.56 ± 0.08
    ATP-dependent Clp protease regulatory subunitclpCsll00201.91 ± 0.241.7 ± 0.42.61 ± 0.712.3 ± 0.24
    Carboxyl-terminal processing proteasectpAslr00081.97 ± 0.541.6 ± 0.463.59 ± 1.402.03 ± 0.73
    Protease HhoAhhoAsll16791.28 ± 0.291.25 ± 0.512.18 ± 0.422.66 ± 0.3
    Protease HhoBhhoBsll14271.37 ± 0.251.29 ± 0.062.08 ± 0.402.18 ± 0.24
    Carboxyl-terminal proteaseprcslr17511.2 ± 0.311.21 ± 0.411.79 ± 0.432.5 ± 0.59
    Elongation factor EF-Gfusslr11051.3 ± 0.371.55 ± 0.381.42 ± 0.482.31 ± 0.58
    Methionine aminopeptidasemapslr09181.2 ± 0.341.47 ± 0.362.05 ± 0.821.05 ± 0.23
    Ribosomal-protein-alanine acetyltransferaserimlslr08532.17 ± 1.071.26 ± 0.222.97 ± 0.381.48 ± 0.42
Transport and binding proteins
    Ferrichrome-iron receptorfhuAsll14090.93 ± 0.150.86 ± 0.130.82 ± 0.172.73 ± 0.51
    ABC transporter subunitycf24slr00740.85 ± 0.151.37 ± 0.383.27 ± 1.561.19 ± 0.42
    ABC transportersll07591.54 ± 0.52.23 ± 0.483.32 ± 1.532.57 ± 0.87
    NaH-antiporter proteinsll06891.00 ± 0.130.9 ± 0.090.92 ± 0.152.79 ± 0.56
    Periplasmic iron-binding proteinslr05130.91 ± 0.120.85 ± 0.140.83 ± 0.062.97 ± 1.09
    ABC-transporter membrane fusion proteinsll14811.45 ± 0.61.37 ± 0.473.19 ± 0.952.29 ± 0.29
Other categories
    High-light-inducible proteinhliAssl25424.07 ± 0.662.53 ± 1.894.54 ± 0.741.71 ± 0.11
    High-light-inducible proteinhliBssl25957.33 ± 0.812.94 ± 0.5631.00 ± 6.001.88 ± 0.62
    High-light-inducible proteinhliCssl16333.43 ± 0.92.24 ± 1.008.23 ± 1.633.73 ± 0.83
    Acriflavin resistance proteinacrFslr21311.69 ± 0.311.45 ± 0.132.02 ± 0.093.29 ± 0.54
    Quinolene resistance protein NorAnorAsll11541.48 ± 0.181.53 ± 0.344.09 ± 0.591.91 ± 0.28
    Hydrogenase expression/formation proteinhypAslr16753.77 ± 0.921.55 ± 0.448.28 ± 1.182.24 ± 1.01
    Probable ferredoxinssl30442.36 ± 0.991.71 ± 0.354.63 ± 2.981.79 ± 0.27
    Secreted protein MPB70sll17350.98 ± 0.380.8 ± 0.190.81 ± 0.042.21 ± 0.68
    Transforming growth factor-induced proteinsll14834.96 ± 1.54.13 ± 0.7721.68 ± 2.3521.97 ± 5.14
    Esteraseslr19162.88 ± 0.481.31 ± 0.372.95 ± 0.540.96 ± 0.31
    Peroxiredoxin family proteinsll16211.34 ± 0.281.47 ± 0.53.85 ± 2.105.32 ± 1.75
    ABC1-like proteinsll17701.98 ± 0.272.23 ± 0.412.34 ± 0.872.29 ± 0.69
    Zinc-containing alcohol dehydrogenase familyslr11921.44 ± 0.221.2 ± 0.32.59 ± 0.421.23 ± 0.22
    Lignostilbene-α,β-dioxygenasesll15412.29 ± 0.771.65 ± 0.656.32 ± 1.803.55 ± 1.04
    CP12 polypeptidessl33641.82 ± 0.441.34 ± 0.312.13 ± 0.211.26 ± 0.11
Hypothetical proteins
    Ycf21ycf21sll17971.55 ± 0.281.33 ± 0.332.76 ± 0.531.93 ± 0.26
    Ycf34ycf34ssr14251.56 ± 0.271.34 ± 0.651.97 ± 0.451.46 ± 0.53
    Ycf38ycf38sll07601.48 ± 0.531.32 ± 0.261.79 ± 0.812.16 ± 0.66
    Putative arsenical pump-driving ATPasesll00861.72 ± 0.711.11 ± 0.552.8 ± 0.582.15 ± 1.06
sll00881.14 ± 0.271.32 ± 0.751.97 ± 0.471.03 ± 0.17
sll01411.61 ± 0.451.63 ± 0.932.45 ± 1.483.2 ± 0.91
sll01571.62 ± 0.41.8 ± 1.013.31 ± 0.811.82 ± 0.95
sll01852.03 ± 0.471.81 ± 0.334.85 ± 2.813.19 ± 0.98
    Potential FMN proteinsll02171.08 ± 0.131.14 ± 0.243.13 ± 0.841.22 ± 0.23
sll02971.42 ± 0.231.59 ± 0.183.05 ± 0.261.64 ± 0.28
sll03141.42 ± 1.161.14 ± 0.272.17 ± 0.672.42 ± 0.97
sll03551.37 ± 0.481.42 ± 0.372.35 ± 0.961.32 ± 0.33
sll04701.22 ± 0.141.06 ± 0.231.77 ± 0.722.61 ± 0.21
sll05287.35 ± 2.873.27 ± 3.2432.54 ± 7.822.23 ± 0.28
sll05491.6 ± 0.471.5 ± 0.323.78 ± 1.241.78 ± 0.17
sll07611.37 ± 0.441.26 ± 0.221.42 ± 0.62.13 ± 0.43
sll08142.09 ± 0.451.52 ± 0.123.28 ± 1.342.71 ± 1.02
sll08465.62 ± 1.011.82 ± 1.6910.66 ± 3.291.43 ± 0.22
sll08981.53 ± 0.431.53 ± 0.312.07 ± 0.661.62 ± 0.5
sll09391.93 ± 0.51.3 ± 0.193.00 ± 1.231.65 ± 0.35
sll10221.8 ± 0.371.29 ± 0.333.1 ± 0.871.03 ± 0.19
sll10711.71 ± 0.512.2 ± 0.493.4 ± 1.31.43 ± 0.48
sll11061.73 ± 0.270.89 ± 0.143.73 ± 1.191.34 ± 0.17
sll11501.07 ± 0.121.11 ± 0.081.99 ± 0.721.6 ± 0.5
sll11551.25 ± 0.211.26 ± 0.252.07 ± 0.511.52 ± 0.17
sll12011.24 ± 0.21.68 ± 0.512.49 ± 0.781.2 ± 0.21
sll12681.37 ± 0.240.95 ± 0.161.3 ± 0.32.1 ± 0.43
sll12891.33 ± 0.521.46 ± 0.443.02 ± 1.641.7 ± 0.54
sll14261.21 ± 0.141.12 ± 0.161.57 ± 0.132.4 ±0.35
sll15151.28 ± 0.32.74 ± 1.292.72 ± 0.732.07 ± 0.58
sll16201.13 ± 0.291.24 ± 0.242.1 ± 0.80.98 ± 0.15
sll17221.02 ± 0.411.42 ± 0.812.19 ± 1.782.27 ± 0.65
sll17691.64 ± 0.332.08 ± 0.473.3 ± 1.261.7 ± 0.42
sll17741.38 ± 0.411.64 ± 0.532.47 ± 1.171.24 ± 0.3
sll18841.84 ± 0.471.42 ± 0.33.84 ± 1.911.28 ± 0.32
sll18911.07 ± 0.181.02 ± 0.161.7 ± 0.492.01 ± 0.62
sll19112.08 ± 0.321.56 ± 0.371.9 ± 0.491.62 ± 0.11
sll20131.5 ± 0.831.39 ± 0.182.09 ± 0.710.98 ± 0.24
slr00060.96 ± 0.290.99 ± 0.391.42 ± 0.772.28 ± 0.74
slr01121.18 ± 0.191.21 ± 0.132.76 ± 0.441.92 ± 0.39
slr02701.42 ± 0.31.39 ± 0.52.67 ± 0.381.11 ± 0.11
slr02921.44 ± 0.181.42 ± 0.492.45 ± 0.511.42 ± 0.31
slr03202.62 ± 0.682.74 ± 0.676.55 ± 2.362.68 ± 0.86
slr03641.64 ± 0.361.68 ± 0.353.18 ± 0.871.28 ± 0.18
slr05512 ± 0.732.4 ± 0.882.32 ± 1.121.15 ± 0.41
slr05821.28 ± 0.541.2 ± 0.432.47 ± 0.811.73 ± 0.32
slr05941.18 ± 0.261.16 ± 0.172.48 ± 1.071.35 ± 0.37
slr06421.62 ± 0.271.84 ± 0.643.66 ± 1.022.07 ± 0.36
slr08522.23 ± 1.61.26 ± 0.383.67 ± 1.271.82 ± 0.77
slr08631.53 ± 0.251.39 ± 0.492.00 ± 0.721.09 ± 0.14
slr09593.1 ± 0.851.77 ± 0.734.29 ± 1.071.5 ± 0.12
slr09671.94 ± 0.21.65 ± 0.346.34 ± 1.832.77 ± 0.5
slr10740.99 ± 0.381.26 ± 0.210.46 ± 0.343.69 ± 0.79
slr11191.82 ± 0.481.52 ± 0.833.27 ± 0.881.67 ± 0.58
    Similar to chlorobenzene dioxygenaseslr12051.03 ± 0.291.06 ± 0.280.97 ± 0.299.23 ± 3.29
    slr12351.47 ± 0.271.5 ± 0.282.35 ± 0.131.41 ± 0.14
    slr12591.24 ± 0.211.57 ± 0.453.31 ± 1.590.97 ± 0.17
    Similar to to phytoene dehydrogenaseslr12931.37 ± 0.371.15 ± 0.372.03 ± 0.430.97 ± 0.23
    Putative modulator of DNA gyraseslr13221.31 ± 0.291.21 ± 0.181.98 ± 0.711.86 ± 0.35
slr13831.27 ± 0.561.48 ± 0.542.41 ± 0.691.57 ± 0.48
slr13971.23 ± 0.351.24 ± 0.362.15 ± 1.021.21 ± 0.4
slr14132.7 ± 0.551.53 ± 0.436.27 ± 2.441.43 ± 0.48
slr15121.29 ± 0.351.1 ± 0.231.1 ± 0.8611.63 ± 3.93
slr15131.1 ± 0.171.05 ± 0.161.71 ± 1.513.68 ± 0.66
slr15446.54 ± 2.573.3 ± 2.5617.60 ± 5.102.73 ± 0.56
slr15571.46 ± 0.321.44 ± 0.282.38 ± 1.121.45 ± 0.41
slr16033.64 ± 0.661.79 ± 0.7110.53 ± 3.771.68 ± 0.36
slr16743.9 ± 1.111.91 ± 0.3913.47 ± 2.892.71 ± 0.83
slr16762.58 ± 0.491.74 ± 0.464.16 ± 2.362.53 ± 1.05
slr16772.47 ± 0.481.87 ± 0.412.83 ± 1.22.13 ± 0.6
slr16863.11 ± 1.291.84 ± 0.435.47 ± 3.031.59 ± 0.44
    Cell death suppressor protein Lls1 homologslr17472.1 ± 0.411.85 ± 0.913.32 ± 0.392.64 ± 0.56
    Probable DNA-binding stress proteinslr18941.57 ± 0.241.42 ± 0.234.79 ± 1.922.44 ± 0.55
    slr19152.18 ± 1.10.9 ± 0.161.61 ± 0.911.5 ± 1.32
    slr19171.55 ± 0.441.27 ± 0.482.28 ± 0.640.76 ± 0.15
    slr19261.51 ± 0.141.23 ± 0.242.24 ± 0.940.94 ± 0.33
    slr19301.09 ± 0.181.25 ± 0.422.3 ± 1.151.12 ± 0.31
    Water-soluble carotenoid proteinslr19632.59 ± 0.991.88 ± 0.726.36 ± 2.452.33 ± 0.85
slr19711.36 ± 0.411.05 ± 0.342.17 ± 0.642.00 ± 0.65
slr19901.7 ± 0.271.61 ± 0.262.81 ± 0.50.87 ± 0.04
slr20480.93 ± 0.181.18 ± 0.571.37 ± 0.653.57 ± 1.17
slr20711.01 ± 0.281.01 ± 0.221.97 ± 0.491.29 ± 0.14
ssl02421.5 ± 0.491.3 ± 0.452.05 ± 0.731.19 ± 0.39
ssl29712.54 ± 0.371.24 ± 0.241.65 ± 0.871.38 ± 0.67
ssl34461.71 ± 0.511.31 ± 0.363.93 ± 1.510.95 ± 0.07
ssl37691.25 ± 0.131.32 ± 0.112.82 ± 1.451.23 ± 0.43
ssr03491.11 ± 0.31.09 ± 0.22.72 ± 0.971.12 ± 0.2
ssr12561.27 ± 0.261.18 ± 0.272.01 ± 0.311.36 ± 0.39
ssr20161.81 ± 0.411.64 ± 1.22.77 ± 0.691.32 ± 0.39
ssr31882.45 ± 0.521.24 ± 0.173.03 ± 1.171.15 ± 0.36
  • a Synechocystis gene transcripts induced more than twofold by UV-B or high-intensity white light irradiation. Average expression ratios (treated/control ratio explained in Materials and Methods) and standard deviations from four experiments are included. The treatments were as follows: 20 microeinsteins of UV-B m−2 s−1 (UV low) for 20 min or 2 h; 60 microeinsteins of UV-B m−2 s−1 (UV high) for 2 h; and 200 microeinsteins of intense white light m−2 s−1 for 2 h. Standard deviations were calculated from eight independent measurements of duplicate RNA samples (20 microeinsteins of UV-B m−2 s−1 for 20 min or for 2 h) or four independent measurements of single RNA samples (60 microeinsteins of UV-B m−2 s−1 for 2 h and 200 microeinsteins of white light m−2 s−1 for 2 h). Genes with expression levels unchanged (expression ratio between 2 and 0.5 or difference between ratio and standard deviation is less than 1.0) in all four experiments are not included in the tables. Transcript levels that were induced more than twofold are shown in bold type.