TABLE 2.

Effect of duration of starvation of P. putida cells on spectrum of Phe+ mutations

Day of appearance of mutantsNo. of mutants analyzedNo. (%) of mutants with:
C-to-A transversionG-to-T transversionDeletionsInsertions (2 to 3 nt)Undefined mutations
6 to 45 ntb2 to 3 nt
2a7740 (52)14 (18)16 (21)2 (2.5)3 (4)2 (2.5)
36947 (68)4 (6)4 (6)02 (3)12 (17)
46230 (48)10 (16)2 (3)5 (8)3 (5)12 (20)
57030 (43)9 (13)3 (4)6 (9)022 (31)
66018 (30)12 (20)013 (22)1 (2)16 (26)
76116 (26)11 (18)012 (20)5 (8)17 (28)
  • a Phe+ mutants appearing on selective plates on day 2 contained mutations that occurred in growing cultures before plating.

  • b nt, nucleotides.